Functional molecule and manufacturing method therefor

• US 20110151510A1
• Filed: 01/19/2011
• Published: 06/23/2011
• Est. Priority Date: 03/28/2006
• Status: Active Grant

• Current Assignee: Apta Biosciences Ltd.
• Original Assignee: Fujitsu Limited
• Inventors: Kichise, Tomoyasu, Fujihara, Tsuyoshi, Fujita, Shozo
• Granted Patent: US 9,512,171 B2
• Time in Patent Office: Days
• US Class Current: 435/68.1
• CPC Class Codes: C07K 1/04 on carriers C07K1/003, C07K1/006 take precedence
• CPC Class Codes: C07K 1/1077 by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
• CPC Class Codes: C07K 1/22 Affinity chromatography or related techniques based upon selective absorption processes

Abstract

A novel method for efficiently manufacturing a functional molecule with affinity for a target substance is provided along with a functional molecule manufactured by this method. The method for manufacturing a functional molecule includes binding, to a resin, a specific substance having a cleavable linker which is a site that is cleavable under a specific condition to prepare a specific-substance-bound resin, treating this specific-substance-bound resin with a solution comprising functional molecule candidates, and then cleaving the cleavable linker under the specific condition to select a functional molecule which has interacted specifically with the specific substance.

Claims

1. A method for manufacturing a functional molecule, comprising:

binding, to a resin, a specific substance having a cleavable linker which is a site that is cleavable under a specific condition to prepare a specific-substance-bound resin;

treating said specific-substance-bound resin with a solution comprising functional molecule candidates; and

cleaving said cleavable linker under said specific condition and selecting a functional molecule which has interacted specifically with said specific substance;

wherein said functional molecule is a modified nucleotide n-mer (n≧

2) comprising a modified nucleoside having a substituent group introduced into a nucleoside constituting a nucleic acid, and

wherein said functional molecule can be transformed into a nucleic acid when said substituent group is removed by alkali treatment.

8. The manufacturing method according to claim 1, wherein said cleavable linker is cleavable at 50°

C. or less and in the pH range of 6.0 to 9.0.

2-7. -7. (canceled)

9-10. -10. (canceled)