PAENIBACILLUS SPP. AND METHODS FOR FERMENTATION OF LIGNOCELLULOSIC MATERIALS

• US 20110151532A1
• Filed: 05/22/2009
• Published: 06/23/2011
• Est. Priority Date: 05/23/2008
• Status: Active Grant

• Current Assignee: The University of Georgia Research Foundation, Inc.
• Original Assignee: United of Georgia Research Foundation Incorporated
• Inventors: DeCrescenzo-Henriksen, Emily, Doran Peterson, Joy
• Granted Patent: US 8,652,819 B2
• Time in Patent Office: Days
• US Class Current: 435/162
• CPC Class Codes: C12P 1/04 by using bacteria
• CPC Class Codes: C12P 39/00 Processes involving microorganisms of different genera in the same process, simultaneously
• CPC Class Codes: C12P 7/06 Ethanol, i.e. non-beverage
• CPC Class Codes: C12P 7/065 with microorganisms other than yeasts
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

Provided herein are methods for producing a fermentation product, such as ethanol, by co-culture of a member of the genus Paenibacillus and an ethanologenic microbe, such as yeast or E. coli. Also provided are methods for making enzymes useful in the saccharification of a pretreated lignocellulosic material. The enzymes may be made by culturing a member of the genus Paenibacillus in a composition suitable for production of such enzymes. An example of such a composition is a pretreated lignocellulosic material, for example, spent hydrolysates. Also provided are genetically modified members of the genus Paenibacillus that have been genetically modified to not produce an antimicrobial, for instance, a polymyxin E.

Claims

1. A method for producing ethanol comprising:

fermenting a composition comprising a pretreated lignocellulosic material, wherein the fermenting comprises contacting the composition with an ethanologenic yeast and a Paenibacillus spp.

5. The method of claim 1 wherein the yeast is Saccharomyces cerevisiae.

7. The method of claim 1 wherein the pretreated lignocellulosic material is pine.

9. The method of claim 1 wherein the Paenibacillis spp. is P. amylolyticus.

10. The method of claim 1 wherein the Paenibacillis spp. produces an enzyme having saccharifying activity when incubated on a medium comprising inorganic salts and a carbon source selected from glucose, mannose, xylose, arabinose, cellulose, pectin, starch, xylan, carboxymethylcellulose, or a combination thereof.

2-4. -4. (canceled)

6. (canceled)

8. (canceled)

11-18. -18. (canceled)

19. (canceled)

20. A method comprising:

providing a composition comprising spent hydrolysates;

culturing a Paenibacillus spp. in the composition under conditions suitable for the production of an enzyme having saccharifying activity.

24. The method of claim 20 wherein the Paenibacillus amylolyticus is genetically modified to not produce an antimicrobial.

25. The method of claim 20 wherein the spent hydrolysates are obtained from feimentation of a pretreated lignocellulosic material.

27. The method of claim 20 wherein the culturing results in a second composition comprising the Paenibacillis spp. and an enzyme having saccharifying activity, the method further comprising mixing the second composition with a composition comprising a pretreated lignocellulosic material to result in a fermentation composition.

28. The method of claim 27 further comprising contacting the fermentation composition with an enthanologenic microbeyeast.

30. The method of claim 27 wherein the yeast is Saccharomyces cerevisiae.

21-23. -23. (canceled)

26. (canceled)

29. (canceled)

31. (canceled)

32. A method comprising:

providing a first composition obtained from culturing a Paenibacillus spp. in a second composition comprising spent hydrolysates under conditions suitable for the production of an enzyme having saccharifying activity; and

mixing the first composition with a third composition comprising a pretreated lignocellulosic material to result in a fermentation composition.

33. The method of claim 32 further comprising contacting the fermentation composition with an ethanologenic yeast.

37. The method of claim 32 wherein the Paenibacillis spp. is P. amylolyticus.

34. (canceled)

35. The method of 32 wherein the yeast is Saccharomyces cerevisiae.

36. (canceled)

38-40. -40. (canceled)

41. A method comprising:

culturing a Paenibacillus spp. in a composition comprising spent hydrolysates under conditions suitable for the production of an enzyme having saccharifying activity, wherein the culturing results in a second composition comprising the Paenibacillis spp. and an enzyme having saccharifying activity.

42. The method of claim 41 further comprising substantially removing the Paenibacillis spp. from the second composition.

43. The method of claim 41 wherein the Paenibacillis spp. is P. amylolyticus.

46. The method of claim 43 wherein the Paenibacillus amylolyticus is genetically modified to not produce an antimicrobial.

44-45. -45. (canceled)

47. A genetically modified Paenibacillus amylolyticus that has been genetically modified to not produce an antimicrobial.

48-49. -49. (canceled)