DUAL INLET MICROCHANNEL DEVICE AND METHOD FOR USING SAME
First Claim
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1. A method for performing a kinetic assay, the method comprising the steps of:
- attaching a fluid handling system to a device, where the fluid handling system includes a first fluid delivery tip, a second fluid delivery tip, and a fluid removal tip all of which are connected to the device, where the device has a body including;
a first fluid inlet connected to the first fluid delivery tip;
a second fluid inlet connected to the second fluid delivery tip;
a fluid outlet connected to the fluid removal tip;
a flow chamber including;
a first flow restrictor associated with the first fluid inlet;
a second flow restrictor associated with the second fluid inlet;
a central portion having one corner of one end associated with the first flow restrictor and another corner of the one end associated with the second flow restrictor;
the central portion having a sensing surface on which there is immobilized a target; and
an outlet portion associated with fluid outlet and an opposite end of the central portion, wherein the opposite end is directly opposite from both an opening of the one corner and an opening of the another corner associated with the first end of the central portion; and
flowing a buffer solution through the first fluid delivery tip and into and through the flow chamber and then out the fluid removal tip while an inspection system obtains a baseline measurement;
stopping the flow of the buffer solution;
flowing an analyte solution through the second fluid delivery tip and into and through the flow chamber and then out the fluid removal tip while the inspection system obtains an association measurement;
stopping the flow of the analyte solution; and
flowing the buffer solution through the first fluid delivery tip and into and through the flow chamber and then out the fluid removal tip while the inspection system obtains a disassociation measurement.
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Abstract
A dual inlet microchannel device and a method for using the device to perform a flow-through kinetic assay are described. A microplate having an array of the dual inlet microchannel devices and in particular their specially configured flow chambers is also described. Several embodiments of the dual inlet microchannel devices and specially configured flow chambers are also described.
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Citations
18 Claims
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1. A method for performing a kinetic assay, the method comprising the steps of:
attaching a fluid handling system to a device, where the fluid handling system includes a first fluid delivery tip, a second fluid delivery tip, and a fluid removal tip all of which are connected to the device, where the device has a body including; a first fluid inlet connected to the first fluid delivery tip; a second fluid inlet connected to the second fluid delivery tip; a fluid outlet connected to the fluid removal tip; a flow chamber including; a first flow restrictor associated with the first fluid inlet; a second flow restrictor associated with the second fluid inlet; a central portion having one corner of one end associated with the first flow restrictor and another corner of the one end associated with the second flow restrictor; the central portion having a sensing surface on which there is immobilized a target; and an outlet portion associated with fluid outlet and an opposite end of the central portion, wherein the opposite end is directly opposite from both an opening of the one corner and an opening of the another corner associated with the first end of the central portion; and flowing a buffer solution through the first fluid delivery tip and into and through the flow chamber and then out the fluid removal tip while an inspection system obtains a baseline measurement; stopping the flow of the buffer solution; flowing an analyte solution through the second fluid delivery tip and into and through the flow chamber and then out the fluid removal tip while the inspection system obtains an association measurement; stopping the flow of the analyte solution; and flowing the buffer solution through the first fluid delivery tip and into and through the flow chamber and then out the fluid removal tip while the inspection system obtains a disassociation measurement. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A microplate, comprising:
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an upper plate including a top surface, a body, and a bottom surface, where; the top surface has located thereon a sealing substance which has one or more fluid delivery-removal sealing interfaces where each fluid delivery-removal sealing interface has at least two fluid inlet ports and at least one fluid outlet port; and the body having one or more fluid delivery-removal channels extending therethrough where each fluid delivery-removal channel has at least two fluid inlet channels and at least one fluid outlet channel which are respectively aligned with the at least two fluid inlet ports and the at least one fluid outlet port that are located within the corresponding fluid delivery-removal sealing interface of the sealing substance; and a lower plate including a top surface which is attached to the bottom surface of the upper plate such that one or more flow chambers are present therebetween, where each one of the flow chambers is in fluid communication with a corresponding one of the fluid delivery-removal channels extending through the body of the upper plate and where each of the flow chambers further includes; a first flow restrictor associated with one of the corresponding fluid inlet ports; a second flow restrictor associated with another one of the corresponding fluid inlet ports; a central portion having one corner of one end associated with the first flow restrictor and another corner of the one end associated with the second flow restrictor; and an outlet portion associated with the corresponding fluid outlet port and an opposite end of the central portion, wherein the opposite end is directly opposite from both an opening of the one corner and an opening of the another corner associated with the one end of the central portion. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18)
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Specification