Methods for Identifying Subjects With an Increased Likelihood of Responding to DPP-IV Inhibitors
First Claim
1. A method comprising the steps of(a) assaying a sample from an individual with Type 2 diabetes and(b) detecting a reference or a variant allele at one or more polymorphic loci of SEQ ID NOs:
- 1-12, 63, and/or 69-75.
3 Assignments
0 Petitions
Accused Products
Abstract
The invention provides novel in vitro diagnostic methods for identifying subjects or patients who may have an increased likelihood of responding to DPP-IV inhibitor therapy. The invention also provides novel polynucleotides associated with increased responsiveness of a patient to DPP-IV inhibition. Polynucleotide fragments which comprise at least one polymorphic locus, are also provided. Allele-specific primers and probes which hybridize to these polymorphic regions, and/or which comprise at least one polymorphic locus are also provided. The polynucleotides, primers, and probes of the invention are useful in phenotype correlations, medicine, and genetic analysis.
10 Citations
16 Claims
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1. A method comprising the steps of
(a) assaying a sample from an individual with Type 2 diabetes and (b) detecting a reference or a variant allele at one or more polymorphic loci of SEQ ID NOs: - 1-12, 63, and/or 69-75.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 14)
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10. A method of treating an individual with Type 2 diabetes comprising the steps of:
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(a) obtaining a nucleic acid sample from said individual; (b) subjecting the sample to genetic sequencing, microarray, or PCR analysis; (c) displaying sequencing data on a data screen for analysis; (d) recording the identity of said reference or variant allele at one or more polymorphic loci of SEQ ID NOs;
1-12, 63, and/or 69-75;(e) incorporating the identified reference or variant allele data at one or more polymorphic loci of SEQ ID NOs;
1-12, 63, and/or 69-75 into an ANCOVA analysis;(f) conducting an ANCOVA analysis of said reference or variant allele data; (g) determining a probability of the likelihood of a favorable response or the magnitude of a favorable change in HbA1C of said individual resulting from treating said individual with a DPP-IV inhibitor or a DPP-IV inhibitor in combination with other oral antidiabetic therapy; and (h) administering a DPP-IV inhibitor or a DPP-IV inhibitor in combination with other oral antidiabetic therapy to said individual where said individual is determined to have a high probability of likelihood of a favorable response or a significantly high magnitude of a favorable change in HbA1C resulting from treatment of said individual with a DPP-IV inhibitor or a DPP-IV inhibitor in combination with other oral antidiabetic therapy. - View Dependent Claims (12, 13, 15)
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11. A method of treating an individual with Type 2 diabetes comprising the steps of:
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(a) obtaining a nucleic acid sample from said individual; (b) subjecting the sample to sequence analysis; (d) recording the identity of said reference or variant allele at one or more polymorphic loci of SEQ ID NOs;
1-12, 63, and/or 69-75;(e) determining a probability of the likelihood of a favorable response or the magnitude of a favorable change in HbA1C of said individual resulting from treating said individual with a DPP-IV inhibitor or a DPP-IV inhibitor in combination with other oral antidiabetic therapy; and (f) administering a DPP-IV inhibitor or a DPP-IV inhibitor in combination with other oral antidiabetic therapy to said individual where said individual is determined to have a high probability of likelihood of a favorable response or a significantly high magnitude of a favorable change in HbA1c resulting from treatment of said individual with a DPP-IV inhibitor or a DPP-IV inhibitor in combination with other oral antidiabetic therapy.
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16. A diagnostic kit for identifying a subject or patient more likely to have a favorable response to the administration of a therapeutically-effective amount of a DPP-IV inhibitor or a DPP-IV inhibitor in combination with other oral antidiabetic therapy, wherein said kit comprises:
oligonucleotides having SEQ ID NOs;
13-44, 65-66, and 76-103.
Specification