Plant Transcription Factors

• US 20110162101A1
• Filed: 12/16/2010
• Published: 06/30/2011
• Est. Priority Date: 10/24/2000
• Status: Active Grant

• Current Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Original Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Inventors: Cahoon, Rebecca E., Klein, Theodore M.
• Granted Patent: US 8,304,608 B2
• Time in Patent Office: Days
• US Class Current: 800/278
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8247 involving modified lipid metabolism, e.g. seed oil composition
• CPC Class Codes: C12N 15/8261 with agronomic (input) traits, e.g. crop yield
• CPC Class Codes: Y02A 40/146 Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

This invention relates to an isolated nucleic acid fragment encoding a transcription factor. The invention also relates to the construction of a chimeric gene encoding all or a portion of the transcription factor, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of accumulated oil in a transformed host cell.

Claims

1. An isolated polynucleotide comprising:

(a) a nucleotide sequence encoding a polypeptide having LEC1-related transcription factor activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO;

2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 90% sequence identity based on the Clustal alignment method, or

(b) the complement of the nucleotide sequence, wherein the complement and the nucleotide sequence contain the same number of nucleotides and are 100% complementary.

2. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:

2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 92% identity based on the Clustal alignment method.

3. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:

2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 94% identity based on the Clustal alignment method.

4. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:

2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 96% identity based on the Clustal alignment method.

5. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:

2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 98% identity based on the Clustal alignment method.

6. The polynucleotide of claim 1, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:

2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30.

7. The polynucleotide of claim 1 wherein the nucleotide sequence comprises the nucleotide sequence of SEQ ID NO:

1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, or 29.

8. A vector comprising the polynucleotide of claim 1.

9. A recombinant DNA construct comprising the polynucleotide of claim 1 operably linked to a regulatory sequence.

10. A method for transforming a cell, comprising transforming a cell the polynucleotide of claim 1.

11. A cell comprising the recombinant DNA construct of claim 9.

12. A method for producing a plant comprising transforming a plant cell with the polynucleotide of claim 1 and regenerating a plant from the transformed plant cell.

13. A plant comprising the recombinant DNA construct of claim 9.

14. A seed comprising the recombinant DNA construct of claim 9.

22. A method for isolating a polypeptide encoded by the polynucleotide of claim 1 comprising isolating the polynucleotide from a cell containing a recombinant DNA construct comprising the polynucleotide operably linked to a regulatory sequence.

15. An isolated polynucleotide comprising a first nucleotide sequence, wherein the first nucleotide sequence contains at least 30 nucleotides, and wherein the first nucleotide sequence is comprised by another polynucleotide, wherein the other polynucleotide includes:

(a) a second nucleotide sequence, wherein the second nucleotide sequence encodes a polypeptide having Lec1-related transcription factor activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO;

2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 90% sequence identity based on the Clustal alignment method, or

(b) the complement of the second nucleotide sequence, wherein the complement and the second nucleotide sequence contain the came number of nucleotides and are 100% complementary.

16. An isolated polypeptide having Lec1-related transcription factor activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:

2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 90% identity based on the Clustal alignment method.

17. The polypeptide of claim 16, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:

2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 92% identity based on the Clustal alignment method.

18. The polypeptide of claim 16, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:

2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 94% identity based on the Clustal alignment method.

19. The polypeptide of claim 16, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:

2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 96% identity based on the Clustal alignment method.

20. The polypeptide of claim 16, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:

2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 98% identity based on the Clustal alignment method.

21. The polypeptide of claim 16, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:

2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30.