INTERFACES THAT ELIMINATE NON-SPECIFIC ADSORPTION, AND INTRODUCE SPECIFIC INTERACTIONS

• US 20110165037A1
• Filed: 01/07/2011
• Published: 07/07/2011
• Est. Priority Date: 01/07/2010
• Status: Abandoned Application

• Current Assignee: University of Chicago
• Original Assignee: University of Chicago
• Inventors: Kreutz, Jason Eugene, Ismagilov, Rustem F.
• Application Number:
  US12/986,777
• Publication Number:
  US 20110165037A1
• Time in Patent Office: Days
• US Class Current: 422/502
• CPC Class Codes: B01L 2200/141 Preventing contamination, tampering
• CPC Class Codes: B01L 3/502784 specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: C07C 271/22 to carbon atoms of hydrocarbon radicals substituted by carboxyl groups
• CPC Class Codes: C07C 41/09 by dehydration of compounds containing hydroxy groups
• CPC Class Codes: C07C 43/13 containing hydroxy or O-metal groups C07C43/11 takes precedence
• CPC Class Codes: C08G 65/007 containing fluorine
• CPC Class Codes: C08G 65/33396 having oxygen in addition to nitrogen
• CPC Class Codes: C08G 65/337 containing other elements organic compounds containing halogens only as halides of a carboxyl group C08G65/332

Abstract

A microfluidic system comprising a microchannel, a carrier fluid comprising a fluorinated oil in the microchannel, at least one plug comprising an aqueous plug-fluid in the microchannel and substantially surrounded on all sides by the carrier-fluid, and a fluorinated surfactant comprising a functional group capable of selectively binding a target molecule is disclosed. A compound for use therewith and a method of synthesizing a fluorinated surfactant are also provided.

Claims

1. A microfluidic system comprising:

a microchannel;

a carrier fluid comprising a fluorinated oil in the microchannel;

at least one plug comprising an aqueous plug-fluid in the microchannel and substantially surrounded on all sides by the carrier-fluid;

a fluorinated surfactant comprising a functional group capable of selectively binding a target molecule.

2. The microfluidic system of claim 1, further comprising a second fluorinated surfactant.

3. The microfluidic system of claim 2, wherein the second fluorinated surfactant comprises a functional group that does not significantly bind to the target molecule.

4. The microfluidic system of claim 1, wherein the target molecule is a biological molecule.

5. The microfluidic system of claim 1, wherein the target molecule is water soluble.

6. The microfluidic system of claim 1, wherein the functional group is selected from the group comprising a nitrilotriacetate, an iminediacetate a triazacyclononane, a biotin derivative, a glutathione derivative, a maltose derivative, an antibody, an aptamer, a thioredoxin tag, a FLAG tag, a hemaglutinin tag, and an OmpA signal sequence tag.

7. A compound of the formula

8. A method of synthesizing a fluorinated surfactant of the formula

CF₃(CF₂)_n(CH₂)_mO(CH₂CH₂O)_pH;

wherein n is an integer from 1 to 20;

m is an integer from 1 to 4;

p is an integer from 3 to 6;

comprising coupling a compound of the formula CF₃(CF₂)_n(CH₂)_mOH with a compound of the formula HO(CH₂CH₂O)_pH.