METHODS OF DIAGNOSING ALZHEIMER'S DISEASE
First Claim
1. A method of diagnosing Alzheimer'"'"'s disease in a subject, the method comprising detecting at least one differentially expressed protein in a tissue sample or body fluid sample from said subject.
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Accused Products
Abstract
Methods and compositions relating to Alzheimer'"'"'s disease are provided. Specifically, proteins that are differentially expressed in the Alzheimer'"'"'s disease state relative to their expression in the normal state are provided. Proteins associated with Alzheimer'"'"'s disease are identified and described. Methods of diagnosis of Alzheimer'"'"'s disease using the differentially expressed proteins are also provided, as are methods for the identification and therapeutic use of compounds for the prevention and treatment of Alzheimer'"'"'s disease.
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Citations
47 Claims
- 1. A method of diagnosing Alzheimer'"'"'s disease in a subject, the method comprising detecting at least one differentially expressed protein in a tissue sample or body fluid sample from said subject.
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4. A method of determining the nature or degree of Alzheimer'"'"'s disease in a human or animal subject, the method comprising:
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(a) establishing a paradigm in which at least one protein is differentially expressed in relevant tissue or body fluid sample from, or representative of, subjects having differential levels of Alzheimer'"'"'s disease; (b) obtaining a sample of the tissue or body fluid from the subject; (c) determining the presence, absence or degree of expression of the differentially expressed protein or proteins in the sample; and (d) relating the determination to the nature or degree of the Alzheimer'"'"'s disease by reference to a previous correlation between such a determination and clinical information. - View Dependent Claims (5, 6, 7, 8, 9, 17, 18, 36, 37)
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23. A method of treatment by the use of an agent that will restore the expression of one or more differentially expressed proteins in the Alzheimer'"'"'s disease state to that found in the normal state in order to prevent the development or progression of Alzheimer'"'"'s disease.
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24. A method of screening an agent to determine its usefulness in treating Alzheimer'"'"'s disease, the method comprising:
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(a) obtaining a sample of relevant tissue or body fluid taken from, or representative of, a subject having Alzheimer'"'"'s disease symptoms, who or which has been treated with the agent being screened; (b) determining the presence, absence or degree of expression of a differentially expressed protein or proteins in the tissue from, or representative of, the treated subject; and
,(c) selecting or rejecting the agent according to the extent to which it changes the expression, activity or amount of the differentially expressed protein or proteins in the treated subject having Alzheimer'"'"'s disease symptoms. - View Dependent Claims (25, 26, 27, 30, 31, 32, 33, 34, 35, 38, 39, 41, 42)
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28. A method of screening an agent to determine its usefulness in treating Alzheimer'"'"'s disease, the method comprising:
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(a) obtaining over time samples of relevant tissue or body fluid taken from, or representative of, a subject having Alzheimer'"'"'s disease symptoms, who or which has been treated with the agent being screened; (b) determining the presence, absence or degree of expression of a differentially expressed protein or proteins in said samples; and
,(c) determining whether the agent affects the change over time in the expression of the differentially expression protein in the treated subject having Alzheimer'"'"'s disease symptoms. - View Dependent Claims (29, 47)
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40. A method according to claim 40, wherein said fragment comprises amino acid residues 270-309 of apolipoprotein A-IV;
- or residues 1446-1744 of complement C4;
or a rodent equivalent thereof.
- or residues 1446-1744 of complement C4;
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43. A method of identifying a protein which is differentially expressed in relevant tissue or body fluid sample from subjects with Alzheimer'"'"'s disease and normal subjects, comprising:
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i) immobilising a tissue sample or body fluid sample or protein-containing extract thereof on a solid support ii) analysing the immobilised proteins by surface enhanced laser desorption time of flight mass spectroscopy iii) comparing the spectra obtained to detect differences in protein expression between Alzheimer'"'"'s subjects and normal subjects. - View Dependent Claims (44, 45, 46)
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Specification