Nanopore and Carbon Nanotube Based DNA Sequencer
First Claim
1. A device for measuring a desired characteristics of a biomolecule comprising a carbon nanotube wherein the biomolecule is translocated through the carbon nanotube.
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Abstract
The present invention provides a device for analyzing the composition of a heteropolymer comprising a carbon nanotube through which the heteropolymer is driven by electrophoresis. The carbon nanotube also serves as one electrode in a reading circuit. One end of the carbon nanotube is held in close proximity to a second electrode, and each end of the carbon nanotube is functionalized with flexibly-tethered chemical-recognition moieties, such that one will bind one site on the emerging polymer, and the second will bind another site in close proximity, generating an electrical signal between the two electrodes when the circuit is completed by the process of chemical recognition.
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Citations
29 Claims
- 1. A device for measuring a desired characteristics of a biomolecule comprising a carbon nanotube wherein the biomolecule is translocated through the carbon nanotube.
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5. A device for determining the sequence of a polymer comprising:
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a) a carbon nanotube having an interior diameter through which the polymer can travel; b) a solid surface to support the carbon nanotube; c) an insulating film layer disposed on top of the carbon nanotube, the insulating film layer comprising at least a first and second well separated by an insulating wall formed by the insulating film layer, and wherein the first and second wells are capable of containing an electrolyte, wherein the first and second well each comprise an electrode or are connected to an electrode; wherein the carbon nanotube comprises a first portion and a second portion, wherein the first portion contacts the first well and the second portion contacts the second well; wherein the second portion has a first part and a second part separated by a gap having a first gap end and a second gap end; wherein the first gap end is functionalized with a first recognition element and the second gap end is functionalized with a second recognition element. wherein the device further comprises a first electrode that contacts the first part of the second portion of the carbon nanotube and comprises a second electrode that contacts the second part of the second portion of the carbon nanotube; wherein the first and second electrodes contacting the second portion of the carbon nanotube are independent of the electrodes contacting the wells in the insulating film layer. - View Dependent Claims (6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A method of fabricating a device suitable for polymer sequencing comprising
a) providing an oxidized silicon wafer support comprising SiO2 and CoMo-doped mesoporous SiO2 catalyst particles patterned on one end of the support; -
b) growing a carbon nanotube via a chemical vapor deposition process from the CoMo-doped mesoporous SiO2 catalyst particles using ethanol as a carbon source to produce the carbon nanotube disposed on top of the silicon wafer; c) cutting a gap in a second portion of the carbon nanotube by oxygen plasma to produce a gap between a first part and a second part of the second portion of the carbon nanotube, wherein the first part has a first gap end and the second part has a second gap end; wherein the oxygen plasma cutting provides a carboxylated first gap end and a carboxylated second gap end; d) providing a first electrode to contact a first part of the second portion of the carbon nanotube, and providing an electrode to contact the second part of the second portion of the carbon nanotube; g) spincasting an insulating layer on the silicon wafer and the carbon nanotube; h) creating a first and second well in the insulating layer so that the first portion of the carbon nanotube contacts the first well and the second portion of the carbon nanotube contacts the second well; and i) contacting the first well with a third electrode and contacting the second well with a fourth electrode, wherein the third and fourth electrodes do not physically contact the carbon nanotube. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27)
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28. A method of fabricating a device suitable for polymer sequencing comprising
a) providing a oxidized silicon wafer support comprising SiO2 and CoMo-doped mesoporous SiO2 catalyst particles patterned on one end of the support; -
b) growing a carbon nanotube via a chemical vapor deposition process from the CoMo-doped mesoporous SiO2 catalyst particles using ethanol as a carbon source to produce the carbon nanotube disposed on top of the silicon wafer; c) providing an aluminum mask over the carbon nanotube wherein the aluminum mask is patterned to expose a section of the carbon nanotube; d) removing the exposed section of the carbon nanotube with an oxygen plasma to expose a bare silicon wafer support, wherein the amount removed is between 1-10 nm, and wherein the non-exposed section of the carbon nanotube is protected from the oxygen plasma etch by the aluminum mask; e) depositing a metal layer over both the aluminum mask and the bare silicon wafer support; f) removing the aluminum mask with tetramethylammonium hydroxide to create a gap between the end of the nanotube and the metal layer. - View Dependent Claims (29)
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Specification