SYSTEM AND METHODS FOR MEASURING BIOMARKER PROFILES

• US 20110172501A1
• Filed: 08/27/2009
• Published: 07/14/2011
• Est. Priority Date: 08/27/2008
• Status: Abandoned Application

• Current Assignee: H. Lundbeck A/S (Lundbeck Foundation)
• Original Assignee: H. Lundbeck A/S (Lundbeck Foundation)
• Inventors: Artymyshyn, Roman, Tamm, Joseph, Vistisen, Jan Bastholm, Antonijevic, Irina, Gerald, Christophe P.G.
• Application Number:
  US13/000,405
• Publication Number:
  US 20110172501A1
• Time in Patent Office: Days
• US Class Current: 600/300
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 2600/158 Expression markers
• CPC Class Codes: G16B 40/00 ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
• CPC Class Codes: G16B 40/20 Supervised data analysis

Abstract

The present invention relates to methods and systems for diagnosing patients with affective disorders. The methods are also useful for predicting the susceptibility for an affective disorder in a subject.

Claims

1. A method of diagnosing an affective disorder in a test subject, the method comprising:

evaluating whether a plurality of features of a plurality of biomarkers in a biomarker profile of the test subject satisfies a value set, wherein satisfying the value set predicts that the test subject has said affective disorder, and wherein the plurality of features are measurable aspects of the plurality of biomarkers, the plurality of biomarkers comprising at least two biomarkers listed in Table 1A.

2. The method of claim 1, the method further comprising outputting a diagnosis of whether the test subject has the affective disorder to a user interface device, a monitor, a tangible computer readable storage medium, or a local or remote computer system;

or displaying a diagnosis of whether the test subject has the affective disorder in user readable form.

3. The method of claim 1, wherein said plurality of biomarkers consists of between 2 and 29 biomarkers listed in Table 1A.

4. The method of claim 1, wherein said plurality of biomarkers consists of between 3 and 20 biomarkers listed in Table 1A.

6. The method of claim 1, wherein said plurality of biomarkers comprises at least three biomarkers listed in Table 1A.

7. The method of claim 1, wherein said plurality of biomarkers comprises at least four biomarkers listed in Table 1A.

11. The method of claim 1, wherein said plurality of biomarkers comprises ERK1 and MAPK14.

12. The method of claim 1, wherein said plurality of biomarkers comprises Gi2 and IL-1b.

13. The method of claim 1, wherein said plurality of biomarkers comprises ARRB1 and MAPK14.

14. The method of claim 1, wherein said plurality of biomarkers comprises ERK1 and IL1b.

15. The method of claim 1, wherein said plurality of biomarkers comprises ARRB1, IL6 and CD8a.

16. The method of claim 1, wherein said plurality of biomarkers comprises ARRB1, ODC1 and P2X7.

17. The method of claim 1, wherein each biomarker in said plurality of biomarkers is a nucleic acid.

18. The method of claim 1, wherein each biomarker is in said plurality of biomarkers is a DNA, a cDNA, an amplified DNA, an RNA, or an mRNA.

19. The method of claim 1, wherein a feature in said plurality of features in the biomarker profile of the test subject is a measurable aspect of a biomarker in the plurality of biomarkers and a feature value for said feature is determined using a biological sample taken from said test subject.

20. The method of claim 19, wherein said feature is abundance of said biomarker in the biological sample, and the biological sample is whole blood.

21. The method of claim 1, the method further comprising constructing, prior to the evaluating step, said first value set.

22. The method of claim 21, wherein the constructing step comprises applying a data analysis algorithm to features obtained from members of a population.

23. The method of claim 22, wherein said population comprises a first plurality of biological samples from a first plurality of control subjects not having the affective disorder and a second plurality of biological samples from a second plurality of subjects having the affective disorder.

24. The method of claim 22, wherein said data analysis algorithm is a decision tree, predictive analysis of microarrays, a multiple additive regression tree, a neural network, a clustering algorithm, principal component analysis, a nearest neighbor analysis, a linear discriminant analysis, a quadratic discriminant analysis, a support vector machine, an evolutionary method, a relevance vector machine, a genetic algorithm, a projection pursuit, or weighted voting.

25. The method of claim 21, wherein the constructing step generates a decision rule and wherein said evaluating step comprises applying said decision rule to the plurality of features in order to determine whether they satisfy the first value set.

26. The method of claim 25, wherein said decision rule classifies subjects in said population as (i) subjects that do not have the affective disorder and (ii) subjects that do have the affective disorder with an accuracy of seventy percent or greater.

27. The method of claim 25, wherein said decision rule classifies subjects in said population as (i) subjects that do not have the affective disorder and (ii) subjects that do have the affective disorder with an accuracy of ninety percent or greater.

28. The method of claim 1, wherein the affective disorder is bipolar disorder I, bipolar disorder II, a dysthymic disorder, a depressive disorder, mild depression, moderate depression, severe depression, atypical depression, melancholic depression, or a borderline personality disorder.

30. A computer program product, wherein the computer program product comprises a computer readable storage medium and a computer program mechanism embedded therein, the computer program mechanism comprising instructions for carrying out the method of claim 1.

5. (canceled)

8-10. -10. (canceled)

29. (canceled)

31-35. -35. (canceled)