Device including altered microorganisms, and methods and systems of use

• US 20110172826A1
• Filed: 05/28/2010
• Published: 07/14/2011
• Est. Priority Date: 12/14/2005
• Status: Abandoned Application

• Current Assignee: Searete LLC (Intellectual Ventures LLC)
• Original Assignee: Searete LLC (Intellectual Ventures LLC)
• Inventors: Gustafson, Kyle, Zartman, Jeremiah James, Amodei, Dario G., Tegreene, Clarence T., Somogyi, Christopher, Jung, Edward K.Y., Bershteyn, Anna, Eckhoff, Philip A., Bao, Xiaoyan Robert, Kim, Lily Yvonne, Wood, Lowell L. JR., Bethke, Brett, Lieberman, Erez, Esvelt, Kevin Michael, Kaminsky, William Michael, Kare, Jordin T., Moitra, Ankur, Bangera, Mahalaxmi Gita, Leuthardt, Eric C.
• Application Number:
  US12/806,637
• Publication Number:
  US 20110172826A1
• Time in Patent Office: Days
• US Class Current: 700/266
• CPC Class Codes: A61K 47/6901 Conjugates being cells, cell fragments, viruses, ghosts, red blood cells or viral vectors
• CPC Class Codes: A61K 9/0024 Solid, semi-solid or solidifying implants, which are implanted or injected in body tissue compositions for intravenous administration, normal injectable solutions or dispersions for, e.g. subcutaneous administration A61K9/0019; brain implants A61K9/0085; (coated) prostheses, catheters or stents A61L
• CPC Class Codes: A61L 2300/258 Genetic materials, DNA, RNA, genes, vectors, e.g. plasmids
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 31/16 Biologically active materials, e.g. therapeutic substances A61L31/047 takes precedence
• CPC Class Codes: C12N 1/36 Adaptation or attenuation of cells

Abstract

Devices, methods, and systems are described for administration to at least one biological tissue of at least one device including at least one altered microorganism. In an embodiment, the altered microorganism includes at least one nucleic acid construct encoding at least one therapeutic agent.

Claims

1. A computer-implemented method comprising:

executing one or more instructions located on a recordable medium for regulating dispensing of at least one treatment device including at least one semi-permeable barrier substantially enclosing at least one auxotrophic microorganism, the at least one auxotrophic microorganism including at least one nucleic acid construct encoding at least one therapeutic agent, the barrier defining an interior region and exterior region;

wherein the device includes at least one metabolite required by the at least one auxotrophic microorganism.

2. The computer-implemented method of claim 1, further comprising generating at least one output to a user readable display.

3. The computer-implemented method of claim 2, wherein the generating the at least one output includes generating at least one graphical illustration of one or more of the at least one auxotrophic microorganism, at least one component thereof, or at least one product thereof;

at least one property of the at least one treatment device;

or at least one property of dispensing the at least one treatment device.

4. The computer-implemented method of claim 2, wherein the generating the at least one output includes generating at least one protocol for generating the at least one auxotrophic microorganism.

5. The computer-implemented method of claim 2, wherein the at least one output includes at least one protocol for administering the at least one treatment device to at least one biological tissue.

6. The computer-implemented method of claim 2, wherein the user includes at least one entity.

7. The computer-implemented method of claim 6, wherein the entity includes at least one person, or computer.

8. The computer-implemented method of claim 2, wherein the user readable display includes a human readable display.

9. The computer-implemented method of claim 2, wherein the user readable display includes one or more active displays.

10. The computer-implemented method of claim 2, wherein the user readable display includes one or more passive displays.

11. The computer-implemented method of claim 2, wherein the user readable display includes one or more of a numeric format, graphical format, or audio format.

12. The computer-implemented method of claim 2, further comprising executing one or more instructions for evaluating at least one biological tissue for one or more indicators prior to, during, or subsequent to administering the at least one treatment device to the at least one biological tissue.

13. The computer-implemented method of claim 12, wherein the evaluating at least one biological tissue for one or more indicators includes evaluating at least one of an assay, image, or gross assessment of the at least one biological tissue prior to, during, or subsequent to administering the at least one treatment device to the at least one biological tissue.

14. The computer-implemented method of claim 13, wherein the assay includes at least one technique including spectroscopy, microscopy, electrochemical detection, polynucleotide detection, histological examination, biopsy analysis, fluorescence resonance energy transfer, electron transfer, enzyme assay, electrical conductivity, isoelectric focusing, chromatography, immunoprecipitation, immunoseparation, aptamer binding, filtration, electrophoresis, immunoassay, or radioactive assay.

15. The computer-implemented method of claim 13, wherein the at least one image includes one or more images acquired by at least one of laser, holography, x-ray crystallography, optical coherence tomography, computer-assisted tomography scan, computed tomography, magnetic resonance imaging, positron-emission tomography scan, ultrasound, x-ray, electrical-impedance monitoring, microscopy, spectrometry, flow cytommetry, radioisotope imaging, thermal imaging, infrared visualization, multiphoton calcium-imaging, photography, or in silico generation.

16. The computer-implemented method of claim 1, further comprising executing one or more instructions for evaluating the at least one biological tissue for one or more indicators relating to one or more of:

administering the at least one treatment device, at least one component thereof, or at least one product thereof;

cell or tissue formation;

cell or tissue growth;

cell or tissue apoptosis;

cell or tissue necrosis;

cell division;

cellular cytoskeletal rearrangement;

cell or tissue secretion;

cell or tissue differentiation;

status of the at least one auxotrophic microorganism of the at least one treatment device;

status of the at least one treatment device;

or status of the at least one therapeutic agent.

17. The computer-implemented method of claim 1, further comprising executing one or more instructions for obtaining genetic sequence information from at least one microorganism isolated from at least one biological tissue.

18. The computer-implemented method of claim 1, further comprising executing one or more instructions for altering the at least one microorganism isolated from the at least one biological tissue.

19. The computer-implemented method of claim 18, further comprising executing one or more instructions for amplifying the at least one microorganism isolated from the at least one biological tissue.

20. The computer-implemented method of claim 18, further comprising executing instructions for replacing the at least one microorganism isolated from the at least one biological tissue subsequent to alteration.

21. The computer-implemented method of claim 18, further comprising executing one or more instructions for predetermining at least one microorganism strain or type for altering to produce at least one therapeutic agent based on at least one feature of at least one biological tissue.

22. The computer-implemented method of claim 21, wherein the at least one feature of the at least one biological tissue includes at least one property of one or more microorganism populations associated with the at least one biological tissue.

23. A computer program product, comprising:

a recordable medium bearing one or more instructions for regulating dispensing of at least one treatment device including an semi-permeable barrier structured to substantially enclose at least one auxotrophic microorganism, and

wherein the at least one auxotrophic microorganism includes at least one nucleic acid construct encoding at least one therapeutic agent.

24. The computer program product of claim 23, wherein the recordable medium includes a computer-readable medium.

25. The computer program product of claim 23, wherein the recordable medium includes a communications medium.

26. The computer program product of claim 23, further comprising one or more instructions for evaluating the at least one biological tissue for one or more indicators prior to, during, or subsequent to administering the at least one treatment device.

27. The computer program product of claim 23, further comprising one or more instructions for evaluating the at least one biological tissue for one or more indicators relating to one or more of:

administering the at least one treatment device, at least one component thereof, or at least one product thereof;

cell or tissue formation;

cell or tissue growth;

cell or tissue apoptosis;

cell or tissue necrosis;

cell division;

cellular cytoskeletal rearrangement;

cell or tissue secretion;

cell or tissue differentiation;

status of the at least one auxotrophic microorganism of the at least one treatment device;

status of the at least one treatment device;

or status of the at least one therapeutic agent.

28. The computer program product of claim 23, wherein the first input includes at least one parameter for generating the at least one auxotrophic microorganism.

29. The computer program product of claim 23, wherein the second input includes at least one parameter for administering the at least one treatment device to at least one biological tissue.

30. The computer program product of claim 23, wherein the output includes at least one protocol for generating the at least one auxotrophic microorganism.

31. The computer program product of claim 23, wherein the output includes at least one protocol for administering the at least one treatment device to the at least one biological tissue.

32. The computer program product of claim 23, wherein the at least one output includes at least one graphical illustration of one or more of the at least one auxotrophic microorganism, at least one component thereof, or at least one product thereof;

at least one property of the at least one treatment device;

or at least one property of dispensing the at least one treatment device.

33. The computer program product of claim 23, wherein the user includes at least one entity.

34. The computer program product of claim 33, wherein the entity includes at least one person, or computer.

35. The computer program product of claim 23, wherein the user readable display includes a human readable display.

36. The computer program product of claim 23, wherein the user readable display includes one or more active displays.

37. The computer program product of claim 23, wherein the user readable display includes one or more passive displays.

38. The computer program product of claim 23, wherein the user readable display includes one or more of a numeric format, graphical format, or audio format.

39. The computer program product of claim 23, further comprising one or more instructions for obtaining genetic sequence information from at least one microorganism isolated from the at least one biological tissue.

40. The computer program product of claim 39, further comprising one or more instructions for altering the at least one microorganism isolated from the at least one biological tissue.

41. The computer program product of claim 39, further comprising one or more instructions for amplifying the at least one microorganism isolated from the at least one biological tissue.

42. The computer program product of claim 39, further comprising one or more instructions for reinstating the at least one microorganism isolated from the at least one biological tissue subsequent to modification.

43. The computer program product of claim 23, further comprising one or more instructions for predetermining at least one microorganism type for altering to produce at least one therapeutic agent based on at least one feature of the at least one biological tissue.

44. The computer program product of claim 43, wherein the at least one feature of the at least one biological tissue includes at least one property of one or more microorganism populations associated with the at least one biological tissue.