REDUCED RISK TOBACCO PRODUCTS AND METHODS OF MAKING SAME
First Claim
Patent Images
1. A method of making a tobacco product with a reduced potential to contribute to a tobacco related disease comprising:
- (a) providing a genetically modified tobacco configured to deliver a reduced amount of a compound that contributes to a tobacco related disease, as compared to a reference tobacco or a conventional tobacco;
(b) contacting a mammalian cell with smoke, or a smoke condensate obtained from said genetically modified tobacco;
(c) identifying a modulation of homeostasis of said cell, as compared to a control cell, which has been contacted with smoke, or a smoke condensate obtained from said reference tobacco or said conventional tobacco, wherein a decreased modulation of homeostasis in said cell compared to modulation of homeostasis in said control cell indicates a reduction in the potential to contribute to a tobacco related disease; and
(d) incorporating said identified genetically modified tobacco into a tobacco product.
7 Assignments
0 Petitions
Accused Products
Abstract
Embodiments provided herein concern tobacco and tobacco products having a reduced amount of a harmful compound. More specifically, several embodiments concern approaches to modify the expression of a gene that is involved in the production of a harmful compound in tobacco, tobacco products made using these approaches and methods of determining whether the removal of said compounds using said approaches yields a tobacco and/or a tobacco product that has a reduced potential to contribute to a tobacco-related disease.
89 Citations
86 Claims
-
1. A method of making a tobacco product with a reduced potential to contribute to a tobacco related disease comprising:
-
(a) providing a genetically modified tobacco configured to deliver a reduced amount of a compound that contributes to a tobacco related disease, as compared to a reference tobacco or a conventional tobacco; (b) contacting a mammalian cell with smoke, or a smoke condensate obtained from said genetically modified tobacco; (c) identifying a modulation of homeostasis of said cell, as compared to a control cell, which has been contacted with smoke, or a smoke condensate obtained from said reference tobacco or said conventional tobacco, wherein a decreased modulation of homeostasis in said cell compared to modulation of homeostasis in said control cell indicates a reduction in the potential to contribute to a tobacco related disease; and (d) incorporating said identified genetically modified tobacco into a tobacco product. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 18, 19, 20, 21, 22)
-
- 14. A tobacco product comprising a genetically modified tobacco that comprises a reduced amount of nicotine as compared to a conventional tobacco product of the same class or a reference tobacco product of the same class and a heterologous nucleic acid that inhibits expression of at least two enzymes involved in nicotine biosynthesis.
-
15. A tobacco product comprising a genetically modified tobacco that comprises a reduced amount of a sterol as compared to a conventional tobacco product of the same class or a reference tobacco product of the same class and a heterologous nucleic acid that inhibits expression of an enzyme involved in sterol biosynthesis.
- 23. A genetically modified tobacco that produces a reduced amount of a compound that contributes to a tobacco related disease, as compared to a conventional tobacco product of the same class or a reference tobacco product of the same class, comprising a heterologous nucleic acid that inhibits expression of an enzyme in the biosynthetic pathway of a compound that contributes to a tobacco related disease.
-
24. A reduced risk tobacco product comprising a genetically modified tobacco that produces a reduced amount of a compound that contributes to a tobacco related disease, as compared to a conventional tobacco product of the same class or a reference tobacco product of the same class.
-
28. A method of making a reduced risk tobacco product comprising:
-
(a) providing a modified tobacco or modified tobacco product configured to deliver to a user a reduced amount of a compound that contributes to a tobacco related disease, as compared to a reference tobacco or tobacco product or a conventional tobacco or tobacco product; (b) contacting smoke or smoke condensate obtained from said modified tobacco or modified tobacco product with a cell; (c) identifying a modulation of homeostasis of said cell, as compared to a control cell, which has been contacted with smoke or a smoke condensate obtained from said reference tobacco or tobacco product or said conventional tobacco or tobacco product, wherein a decreased modulation of homeostasis in said cell compared to modulation of homeostasis in said control cell indicates a reduction in the potential to contribute to a tobacco related disease; and (d) incorporating said modified tobacco or modified tobacco product into said reduced risk tobacco product. - View Dependent Claims (29, 30, 31)
-
-
32. A reduced risk tobacco substantially described herein.
-
33. A reduced risk tobacco product substantially described herein.
-
35. An isolated nucleic acid substantially as described herein.
-
36. An isolated inhibition cassette substantially as described herein.
- 37. A genetically modified tobacco having a reduced amount of nicotine as compared to conventional tobacco, further comprising a heterologous nucleic acid that encodes a gene that produces a composition selected from the group consisting of a medicinal compound, industrial oil, or dietary supplement, wherein said composition is substantially not present in conventional or wild-type tobacco.
- 41. A method of producing a reduced-nicotine tobacco plant, comprising genetically engineering A622 suppression in said plant.
-
48. An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
-
(a) a nucleotide sequence set forth in SEQ ID NO;
5;(b) a nucleotide sequence comprising at least 15 consecutive nucleotides of the nucleotide sequence of SEQ ID NO;
5; and(c) a nucleotide sequence that differs from the nucleotide sequences of (a) or (b) due to degeneracy of the genetic code and encodes a polypeptide with A622 expression, wherein said nucleotide sequence is operatively linked to a heterologous promoter. - View Dependent Claims (49, 50, 51)
-
- 52. A method for reducing an alkaloid in a tobacco plant, comprising suppressing A622.
-
54. A genetically engineered tobacco plant having A622 suppression, wherein said plant exhibits reduced A622 expression compared to a non-transformed control plant and comprises plant cells containing:
(a) a nucleic acid construct comprising in the 5′
to 3′
direction, a promoter operable in said plant cell and a heterologous DNA operably associated with said promoter;
wherein said heterologous DNA is selected from the group consisting of;(i) the DNA sequence of SEQ ID NO;
5; and(ii) a DNA sequence comprising at least 15 consecutive nucleotides of the DNA sequence of SEQ ID NO;
5.- View Dependent Claims (55, 56, 57, 58)
-
59. A method of making a genetically engineered reduced-nicotine tobacco cell having A622 suppression, said method comprising:
- providing a plant cell of a type known to express A622;
providing a nucleic acid construct, comprising, in the 5′
to 3′
direction, a promoter operable in a plant cell and nucleic acid comprising a portion of a sequence encoding A622 mRNA, said nucleic acid operably associated with said promoter; and
transforming said plant cell with said nucleic acid construct to produce transformed cells, wherein said cell has A622 suppression compared to an untransformed cell.
- providing a plant cell of a type known to express A622;
-
61. A method for reducing an alkaloid in a tobacco plant, comprising
(a) suppressing A622; - and
(b) suppressing an additional nicotine biosynthesis enzyme selected from the group consisting of quinolate phosphoribosyl transferase, ornithine decarboxylase, and putrescine N-methyltransferase.
- and
-
62. A method for reducing total alkaloid content in a tobacco plant, comprising
(a) suppressing A622; - and
(b) suppressing an additional nicotine biosynthesis enzyme selected from the group consisting of quinolate phosphoribosyl transferase, ornithine decarboxylase, and putrescine N-methyltransferase. - View Dependent Claims (63, 64, 65)
- and
-
66. A tobacco plant having A622 suppression and reduced total alkaloid content.
-
67. A reduced-nicotine tobacco product that is characterized by a reduced collective amount of N′
- -nitrosonornicotine (NNN), 4-methylnitrosoamino-1-(3-pyridyl)-1butanone (NNK), N′
-nitrosoanatabine (NAT) and N′
-nitrosoanabasine (NAB) and that is prepared from a tobacco plant genetically engineered for A622 suppression, said reduced collective amount of NNN, NNK, NAT and NAB relative to a highly similar tobacco product prepared from a non-transformed control tobacco plant. - View Dependent Claims (68)
- -nitrosonornicotine (NNN), 4-methylnitrosoamino-1-(3-pyridyl)-1butanone (NNK), N′
- 69. A tobacco product having a reduced amount of nitrosoanatabine (NAT) prepared from a tobacco plant having genetically engineered A622 suppression, said amount of NAT being reduced relative to the level in a similar tobacco product prepared from a non-transformed control tobacco plant.
- 71. A reduced-anabasine tobacco product prepared from a tobacco plant having genetically engineered A622 suppression.
-
73. A method for determining the risk potential of a tobacco or tobacco product comprising:
-
(a) providing a tobacco or a tobacco product; (b) obtaining smoke or a smoke condensate from said tobacco or tobacco product; (c) contacting a human cell with smoke or smoke condensate obtained from said tobacco or said tobacco product; and (d) identifying a tobacco-induced cell change in said human cell contacted with said smoke or smoke condensate. - View Dependent Claims (74, 75)
-
-
76. A method of making a cigarette comprising:
-
(a) providing a modified tobacco; (b) contacting a human cell with smoke or smoke condensate obtained from said modified tobacco; (c) identifying a change in homeostasis of said human cell that was contacted with smoke or smoke condensate obtained from said modified tobacco, as compared to a control human cell, which was contacted with smoke or a smoke condensate obtained from a reference tobacco or a conventional tobacco; and (d) incorporating said modified tobacco into a cigarette when a reduced change in homeostasis in said human cell compared to the change in homeostasis in said control human cell is identified. - View Dependent Claims (77, 78, 79, 80, 81)
-
- 82. A cigarette configured to reduce the risk of using said cigarette comprising a reduced risk tobacco and a filter that is designed to reduce the risk associated with cigarette smoke, wherein the reduced risk cigarette configuration and said reduced risk tobacco act synergistically to reduce the change in cell homeostasis.
Specification