Biosensor, thin film electrode forming method, quantification apparatus, and quantification method

• US 20110174613A1
• Filed: 01/11/2011
• Published: 07/21/2011
• Est. Priority Date: 11/15/1999
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Healthcare Company Limited (PHC Holdings Corporation)
• Inventors: Tokunaga, Hiroyuki, Tokuno, Yoshinobu, Yamanishi, Eriko, Fujiwara, Masaki, Miyazaki, Shoji
• Granted Patent: US 8,480,878 B2
• Time in Patent Office: Days
• US Class Current: 204/403.01
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 33/48771 Coding of information, e.g. calibration data, lot number
• CPC Class Codes: Y10T 29/49002 Electrical device making

Abstract

A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.

Claims

1-44. -44. (canceled)

45. A biosensor comprising:

a first support;

a second support which is formed at a position opposite to said first support;

a counter electrode which is formed on said first support;

a measuring electrode and a detecting electrode which are dividedly formed on said second support;

a specimen supply path for introducing a sample liquid between said first support and said second support; and

a reagent layer which is formed in the specimen supply path, wherein said counter electrode has an area equal to or larger than that of said measuring electrode in said specimen supply path.

46. The biosensor as defined in claim 45, wherein there is a space of 0.005 mm to 0.3 mm between the respective electrodes on said second support.

47. The biosensor as defined in claim 45, wherein the thickness of said respective electrode layers is from 3 nm to 100 nm.

48. The biosensor as defined in claim 46, wherein the thickness of said respective electrode layers is from 3 nm to 100 nm.