FLUORESCENT MAGNETIC NANOPARTICLES AND PROCESS OF PREPARATION

• US 20110177339A1
• Filed: 12/16/2010
• Published: 07/21/2011
• Est. Priority Date: 08/08/2003
• Status: Active Grant

• Current Assignee: CapitalBio Corporation, Tsinghua University
• Original Assignee: CapitalBio Corporation, Tsinghua University
• Inventors: Guo, Lianghong, Lu, Huachang, Chen, Depu, Cheng, Jing, Yi, Guangshun
• Granted Patent: US 9,512,001 B2
• Time in Patent Office: Days
• US Class Current: 428/403
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 33/588 with semiconductor nanocrystal label, e.g. quantum dots
• CPC Class Codes: Y10T 428/2991 Coated

Abstract

This invention provides nanometer-sized fluorescent magnetic particles and processes of making them. The nanoparticle has a core particle comprising a magnetic material and a fluorescent material, and the particle size is less than about 1 micrometer. The nanoparticles can be coated with an inorganic or organic layer and can be surface-modified. The nanoparticles can be used in many biological assays.

Claims

1. A nanoparticle comprising a core particle, wherein the core particle comprises a magnetic material and a fluorescent material, and wherein the nanoparticle has a particle size less than about 1 micrometer.

4. The nanoparticle of claim 1, wherein the particle size is less than about 300 nanometers.

6. The nanoparticle of claim 1, wherein the particle size is ranging from about 80 nanometers to about 200 nanometers.

7. The nanoparticle of claim 1, wherein the magnetic material comprises a superparamagnetic, a paramagnetic or a ferromagnetic material.

8. The nanoparticle of claim 1, wherein the magnetic material comprises a metal oxide.

9. The nanoparticle of claim 8, wherein the metal oxide is selected from the group consisting of oxide of cobalt, nickel, manganese, and iron.

12. The nanoparticle of claim 1, wherein the saturation magnetization of the nanoparticle is between about 5 emu/g to about 60 emu/g.

20. The nanoparticle of claim 1, wherein the fluorescent material is a fluorescent manometer-sized particle.

22. The nanoparticle of claim 1, wherein the core particle comprises a magnetic particle covered by a layer of the fluorescent material.

23. The nanoparticle of claim 1, wherein the core particle comprises a fluorescent particle covered by a layer of the magnetic material.

24. The nanoparticle of claim 1, wherein the core particle comprises fluorescent particles doped with the magnetic material.

25. The nanoparticle of claim 1, wherein the core particle comprises magnetic particles doped with the fluorescent material.

26. The nanoparticle of claim 1, wherein the core particle comprises a magnetic particle, a fluorescent particle, and a material to bind the magnetic particle and the fluorescent particle together.

32. The nanoparticle of claim 1, which comprises a bio-molecule.

34. The nanoparticle of claim 32, wherein the bio-molecule is selected from the group consisting of an amino acid, a peptide, a protein, a nucleoside, a nucleotide, an oligonucleotide, a nucleic acid, a vitamin, a monosaccharide, an oligosaccharide, a carbohydrate, a lipid and a complex thereof.

2-3. -3. (canceled)

5. (canceled)

10-11. -11. (canceled)

13-19. -19. (canceled)

21. (canceled)

27-31. -31. (canceled)

33. (canceled)

35-47. -47. (canceled)

48. A process of preparing a nanoparticle comprising fluorescent magnetic particles coated with silica, which process comprises:

a) dispersing nanometer-sized magnetic particles and nanometer-sized fluorescent particles in an alcohol;

b) adding de-ionized water and ammonia having a concentration of 28% to the mixture of step a) at a temperature ranging from about 20°

C. to about 80°

C.; and

c) stirring the mixture of step b) after adding n-ethyl silicate (TEOS) for a period of time ranging from about 0.5 hour to about 8 hours to obtain the nanoparticle.

49. The process of claim 48, wherein the magnetic particles are selected from the group consisting of superparamagnetic, paramagnetic, and ferromagnetic nanometer-sized particles, and nanometer-sized magnetic oxide of cobalt, nickel, and manganese.

52. The process of claim 48, wherein the fluorescent particle is a fluorescein-doped silica particle.

53. The process of claim 48, wherein the surface of the nanoparticle is modified to contain a functional group.

56. The process of claim 48, wherein the nanometer-sized magnetic particles and the nanometer-sized fluorescent particles are dispersed in the alcohol by sonication for a period of time ranging from about 0.5 hour to about 1 hour.

50-51. -51. (canceled)

54-55. -55. (canceled)