Portable Preparation, Analysis, and Detection Apparatus for Nucleic Acid Processing
First Claim
1. A method of sample preparation, comprising:
- loading a biological sample into a cartridge, the cartridge comprising first and second ends, at least one first electrode at the first end, at least one second electrode at the second end, a first sieving matrix disposed between the at least one first electrode and the at least one second electrode, a second sieving matrix disposed between the first sieving matrix and the at least one second electrode and spaced apart from the first sieving matrix;
electroporating the sample by applying a voltage to the at least one first electrode and the at least one second electrode;
moving polar analytes in the sample into the first sieving matrix by an electrically created motive force; and
collecting a portion of the sample resulting from the electrophoretic moving.
0 Assignments
0 Petitions
Accused Products
Abstract
The present teachings comprise a device and method for lysing and/or purifying biological sample. The device can comprise a cartridge having a chamber containing a biological sample receiving region, a plurality of electrodes, and one or more sieving matrices. The electrodes can be configured to lyse the biological sample through the production of a pulsed electrical field. The electrodes can also be configured to heat lyse the biological sample. The electrodes can also be configured to electrophoretically move the biological sample through one or more sieving matrices. A portion of the sample can be isolated on a membrane. The portion of the sample isolated on the membrane can be amplified and detected. A portion of the sample can be isolated in a collection area present in the cartridge. The portion of the sample isolated in the collection area can be removed from the cartridge.
-
Citations
5 Claims
-
1. A method of sample preparation, comprising:
-
loading a biological sample into a cartridge, the cartridge comprising first and second ends, at least one first electrode at the first end, at least one second electrode at the second end, a first sieving matrix disposed between the at least one first electrode and the at least one second electrode, a second sieving matrix disposed between the first sieving matrix and the at least one second electrode and spaced apart from the first sieving matrix; electroporating the sample by applying a voltage to the at least one first electrode and the at least one second electrode; moving polar analytes in the sample into the first sieving matrix by an electrically created motive force; and collecting a portion of the sample resulting from the electrophoretic moving. - View Dependent Claims (2, 3, 4, 5)
-
Specification