Portable Preparation, Analysis, and Detection Apparatus for Nucleic Acid Processing

• US 20110177562A1
• Filed: 12/20/2010
• Published: 07/21/2011
• Est. Priority Date: 11/21/2005
• Status: Abandoned Application

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Vann, Charles S., Chiang, Yuh-Min, Greenstein, Michael
• Application Number:
  US12/973,190
• Publication Number:
  US 20110177562A1
• Time in Patent Office: Days
• US Class Current: 435/91.2
• CPC Class Codes: C12M 47/06 Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material lysis of microorganisms C12N1/06; extracting or separating nucleic acids from biological samples C12N15/1003
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: G01N 27/447 using electrophoresis

Abstract

The present teachings comprise a device and method for lysing and/or purifying biological sample. The device can comprise a cartridge having a chamber containing a biological sample receiving region, a plurality of electrodes, and one or more sieving matrices. The electrodes can be configured to lyse the biological sample through the production of a pulsed electrical field. The electrodes can also be configured to heat lyse the biological sample. The electrodes can also be configured to electrophoretically move the biological sample through one or more sieving matrices. A portion of the sample can be isolated on a membrane. The portion of the sample isolated on the membrane can be amplified and detected. A portion of the sample can be isolated in a collection area present in the cartridge. The portion of the sample isolated in the collection area can be removed from the cartridge.

Claims

1. A method of sample preparation, comprising:

loading a biological sample into a cartridge, the cartridge comprising first and second ends, at least one first electrode at the first end, at least one second electrode at the second end, a first sieving matrix disposed between the at least one first electrode and the at least one second electrode, a second sieving matrix disposed between the first sieving matrix and the at least one second electrode and spaced apart from the first sieving matrix;

electroporating the sample by applying a voltage to the at least one first electrode and the at least one second electrode;

moving polar analytes in the sample into the first sieving matrix by an electrically created motive force; and

collecting a portion of the sample resulting from the electrophoretic moving.

2. The method of claim 1, further comprising lysing the sample by chemical lysing, heating, or sonicating the sample.

3. The method of claim 1, wherein the collecting comprises removing a portion of the sample from the cartridge.

4. The method of claim 1, wherein the detecting comprises visually detecting the emission beams.

5. The method of claim 1, further comprising thermal cycling the biological sample.