GENE EXPRESSION CONTROL IN PLANTS

• US 20110179517A1
• Filed: 03/30/2009
• Published: 07/21/2011
• Est. Priority Date: 04/03/2008
• Status: Active Grant

• Current Assignee: insight genomics limited
• Original Assignee: Vialactia Biosciences (Nz) Limited
• Inventors: Withana, Nimali Piyushika, Templeton, Kerry Robert, Smith-Espinoza, Claudia Jeannette, Phillips, Jonathan Robert, Bajaj, Shivendra, Bryant, Catherine Jane, Puthigae, Sathish
• Granted Patent: US 8,669,108 B2
• Time in Patent Office: Days
• US Class Current: 800/278
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8216 Methods for controlling, regulating or enhancing expression of transgenes in plant cells

Abstract

The invention provides the isolated promoter polynucleotide sequence of SEQ ID NO:1, and fragments and variants thereof. The invention also provides constructs, plant cells and plants genetically modified to contain the promoter polynucleotide. The invention also provides methods for producing plants with altered gene expression and traits via genetic transformation of plants with the promoter polynucleotides.

Claims

1. An isolated promoter polynucleotide comprising:

a) the sequence of SEQ ID NO;

1;

b) a sequence with at least 70% identity to the sequence of SEQ ID NO;

1;

c) at least 50 contiguous nucleotides of the sequence of SEQ ID NO;

1;

d) at least 50 contiguous nucleotides of the sequence of b);

e) a sequence with at least 70% identity to the sequence of c);

or

f) the complement of any one of a) to e).

2. The isolated promoter polynucleotide of claim 1, wherein the promoter polynucleotide is capable of controlling transcription of an operably linked polynucleotide in a plant.

3. The isolated promoter polynucleotide of claim 1, in which the promoter polynucleotide is capable of controlling transcription of an operably linked polynucleotide sequence in at least one of leaves, internodes, roots and flowers.

4. The isolated promoter polynucleotide of claim 1, in which the promoter polynucleotide is capable of driving transcription of an operably linked polynucleotide sequence more prominently in above ground plant parts than in the below ground plant parts.

5. The isolated promoter polynucleotide of claim 1, in which the promoter polynucleotide is capable of driving transcription of an operably linked polynucleotide sequence more prominently in leaves and internodes than in roots and flowers.

6. A genetic construct comprising a promoter polynucleotide of claim 1.

7. The genetic construct of claim 6, in which the promoter polynucleotide is operably linked to a polynucleotide sequence to be expressed.

8. A plant cell or plant transformed with the promoter polynucleotide of claim 1.

9. A plant cell or plant transformed with a genetic construct of claim 6.

10. A method for modifying expression of at least one polynucleotide in a plant cell or plant, the method comprising transformation of the plant cell or plant with a promoter polynucleotide of claim 1.

11. A method for modifying expression of at least one polynucleotide in a plant cell or plant, the method comprising transformation of the plant cell or plant with a genetic construct of claim 6.

12. A method for producing a plant cell or plant with modified expression of at least one polynucleotide, the method comprising:

(a) transforming plant cell or plant with a promoter polynucleotide of claim 1, and

(b) the cultivating the transgenic plant cell or plant under conditions conducive for the promoter polynucleotide to drive transcription.

13. A method for producing a plant cell or plant with modified expression of at least one polynucleotide, the method comprising:

(a) transforming plant cell or plant with a genetic construct of claim 6, and

(b) the cultivating the transgenic plant cell or plant under conditions conducive for the promoter polynucleotide to drive transcription.

14. A method for producing a plant cell or plant with modified expression of at least one gene, the method comprising:

(a) transforming plant cell or plant with a genetic construct of claim 6 wherein the genetic construct comprises a promoter polynucleotide of the invention operably linked to a polynucleotide sequence to be expressed, and

(b) the cultivating the transgenic plant cell or plant under conditions conducive for the promoter polynucleotide to drive transcription of the operably linked sequence.

15. A method for producing a plant cell or plant with a modified phenotype, the method including the stable incorporation into the genome of the plant, a promoter polynucleotide of claim 1.

16. A method for producing a plant cell or plant with a modified phenotype, the method including the stable incorporation into the genome of the plant, a genetic construct of claim 6.

17. A plant cell or plant produced by the method of claim 12.

18. A seed, propagule, progeny, part or product of a plant of claim 8.

19. The seed, propagule, progeny, part or product of claim 18, that contains a polynulceotide comprising:

a) the sequence of SEQ ID NO;

1;

b) a sequence with at least 70% identity to the sequence of SEQ ID NO;

1;

c) at least 50 contiguous nucleotides of the sequence of SEQ ID NO;

1;

d) at least 50 contiguous nucleotides of the sequence of b);

e) a sequence with at least 70% identity to the sequence of c);

or

f) the complement of any one of a) to e).