ENDOPHYTIC FUNGUS AND USES THEREFOR
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Abstract
The present invention provides novel microorganisms, compositions and methods of use thereof, for treating, inhibiting or preventing the developing of a plant pathogenic disease and for killing or inhibiting growth of a variety of pests or pathogens. Provided are compositions comprising a novel endophytic fungal organism effective to inhibit the growth of or kill pests and pathogenic microbes, including Ganoderma boninense. Invention compositions are especially useful in preventing and treating basal stem rot in the oil palm, and can be applied on or in the vicinity of the plant or used to sterilize the plant growth medium prior to or concurrent with plant growth therein. The disclosure further provides substantially purified polynucleotides and polypeptides encoded thereby, together with methods of using those products, for example for making transgenic organism.
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26 Claims
- 1. An isolated culture of a strain of Muscodor strobelii.
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2. An isolated strain of Muscodor strobelii, wherein the strain is Agricultural Research Service Culture Collection accession number NRRL 50288.
- 14. A non-naturally occurring oil palm cultivar that is an oil palm infected with an isolated culture of Muscodor strobelii.
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22. A substantially purified nucleic acid molecule comprising:
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(i) a nucleotide sequence hybridizing under high stringency conditions to any one of the nucleotide sequences in the Sequence Listing, a complement thereof or a fragment of either; (ii) a nucleotide sequence exhibiting a 70% or greater identity to any one of the nucleotide sequences in the Sequence Listing, a complement thereof or a fragment of either; (iii) a nucleotide sequence encoding an amino acid sequence exhibiting a 50% or greater identity to any one of the polypeptides in the Sequence Listing;
or(iv) a nucleotide sequence that is an interfering RNA to a nucleotide sequence according to any one of paragraphs (i)-(iii). - View Dependent Claims (24, 25, 26)
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23. A substantially purified polypeptide, wherein said polypeptide is encoded by a nucleic acid molecule comprising:
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(i) a nucleotide sequence hybridizing under high stringency conditions to any one of the nucleotide sequences in the Sequence Listing, a complement thereof or a fragment of either; (ii) a nucleotide sequence exhibiting a 70% or greater identity to any one of the nucleotide sequences in the Sequence Listing, a complement thereof or a fragment of either;
or(iii) a nucleotide sequence encoding an amino acid sequence exhibiting a 50% or greater identity to any one of the polypeptides in the Sequence Listing.
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Specification