Labelled nucleotides
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Abstract
Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.
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Citations
43 Claims
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1-25. -25. (canceled)
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26. A method for detecting the extension of a primer annealed to a target polynucleotide, comprising:
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(a) providing at least one nucleoside comprising (1) a label attached via a first linker to the nucleoside base, and (2) a protecting group attached via a second linker to the 2′
or 3′
oxygen of the nucleoside sugar,wherein the first and second linkers are both cleavable under a single set of conditions; (b) extending the annealed primer with the nucleoside only if the nucleoside base is complementary to the base at the corresponding position of the target polynucleotide; and (c) detecting the presence of the label on the extended primer; thereby detecting the extension of the primer. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43)
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Specification