Compositions and methods for therapeutic delivery with microorganisms

• US 20110183347A1
• Filed: 01/22/2010
• Published: 07/28/2011
• Est. Priority Date: 01/22/2010
• Status: Active Grant

• Current Assignee: Gearbox LLC (Intellectual Ventures LLC)
• Original Assignee: Searete LLC A Limited Liability Corporation of The State of Delaware
• Inventors: Rinaldo, John D. JR., Lord, Robert W., Jung, Edward K.Y., Levien, Royce A., Wood, Lowell L. JR., Hyde, Roderick A., Malamud, Mark A.
• Granted Patent: US 8,852,916 B2
• Time in Patent Office: Days
• US Class Current: 435/6.13
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 35/74 Bacteria therapeutic use of a bacterial protein A61K38/00
• CPC Class Codes: A61K 35/747 Lactobacilli, e.g. L. acidophilus or L. brevis
• CPC Class Codes: A61K 36/064 Saccharomycetales, e.g. baker's yeast
• CPC Class Codes: A61K 38/162 from virus
• CPC Class Codes: A61K 38/1841 Transforming growth factor [TGF]
• CPC Class Codes: A61K 38/2013 IL-2
• CPC Class Codes: A61K 38/2066 IL-10
• CPC Class Codes: A61K 38/208 IL-12
• CPC Class Codes: C12N 1/36 Adaptation or attenuation of cells
• CPC Class Codes: C12N 15/635 Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

Certain embodiments disclosed relate to compositions, including therapeutic compositions, methods, devices, and systems that include modified microorganisms including at least one genetic element encoding at least one therapeutic agent or environmental treatment agent.

Claims

1. A composition, comprising:

at least one modified microorganism including at least one heterologous genetic element encoding at least one environmental medium treatment agent; and

at least one genetic element inducible to initiate death of the at least one modified microorganism.

2. The composition of claim 1, wherein the at least one modified microorganism further comprises at least one promoter or enhancer operably coupled to the at least one heterologous genetic element encoding at least one environmental medium treatment agent.

3. The composition of claim 2, wherein at least one of the promoter or enhancer operably coupled to the at least one heterologous genetic element encoding at least one environmental medium treatment agent is an epigenetic element.

4. The composition of claim 1, wherein the at least one genetic element inducible to initiate death of the at least one modified microorganism includes at least one heterologous genetic element.

5. The composition of claim 3, wherein at least one of the promoter or enhancer operably coupled to the at least one genetic element inducible to initiate death of the at least one modified microorganism is an epigenetic element.

6. The composition of claim 1, wherein the at least one environmental medium treatment agent includes at least one plant hormone.

7. The composition of claim 6, wherein the at least one plant hormone includes at least one of an auxin, abscisic acid, cytokinin, ethylene, gibberellin, brassinolide, salicyclic acid, jasmonate, polyamine, plant peptide hormone, nitric oxide, strigolactone, or other compound.

8. The composition of claim 1, wherein the at least one genetic element inducible to initiate death of the at least one modified microorganism includes at least one of an inducible promoter, inducible enhancer, or inducible repressor.

19. The composition of claim 1, further comprising at least one metabolite utilizable by the at least one modified microorganism.

20. The composition of claim 19, wherein the at least one metabolite utilizable by the at least one modified microorganism includes at least one inducer of the heterologous genetic element encoding at least one environmental medium treatment agent.

21. The composition of claim 19, wherein the at least one metabolite utilizable by the at least one modified microorganism includes at least one repressor of the at least one heterologous genetic element encoding at least one environmental medium treatment agent.

22. The composition of claim 19, wherein the at least one metabolite utilizable by the at least one modified microorganism includes at least one inducer of the at least one genetic element inducible to initiate death of the at least one modified microorganism.

23. The composition of claim 19, wherein the at least one metabolite utilizable by the at least one modified microorganism includes at least one repressor of the at least one genetic element inducible to initiate death of the at least one modified microorganism.

24. The composition of claim 19, wherein the at least one metabolite utilizable by the at least one modified microorganism includes a time-release formulation.

25. The composition of claim 19, wherein the at least one metabolite uitilizable by the at least one modified microorganism is pH-dependent.

26. The composition of claim 19, wherein the at least metabolite utilizable by the at least one modified microorganism is temperature dependent.

30. The composition of claim 19, wherein the at least one metabolite includes the at least one environmental medium treatment agent.

31. The composition of claim 19, wherein the at least one metabolite is provided or produced by the at least one environmental medium.

32. The composition of claim 19, wherein the at least one metabolite is provided by at least one biological cell.

33. The composition of claim 32, wherein the at least one biological cell includes at least one modified biological cell.

34. The composition of claim 33, wherein the at least one modified biological cell includes at least one prokaryote cell or eukaryote cell.

40. The composition of claim 19, wherein the at least one metabolite includes at least one repressor of the at least one inducible genetic element.

46. The composition of claim 1, wherein the at least one genetic element inducible to initiate death of the at least one modified microorganism includes at least one genetic element inducible to initiate programmed cell death.

52. The composition of claim 1, wherein the at least one modified microorganism includes at least one of a prokaryote or a eukaryote.

53. The composition of claim 1, wherein the at least one modified microorganism includes at least one of bacteria, protozoa, rotifers, algae, archaeon, or fungi.

54. The composition of claim 1, wherein the at least one modified microorganism includes at least one of a non-pathogenic strain, transgenic microorganism, magnetotactic microorganism, anaerobic microorganism or aerobic microorganism, food grade strain, obligate microorganism, attenuated microorganism strain, facultative anaerobe, non-invasive strain, probiotic, colonizing microorganism, element-modifying microorganism, photosynthetic microorganism.

66. The composition of claim 54, wherein the at least one element-modifying microorganism includes at least one of a nitrogen-fixing microorganism, nitrifying microorganism, denitrifying microorganism, hydrocarbon-utilizing microorganism, dechlorinating microorganism, or a sulfate-reducing microorganism.

82. The composition of claim 1, wherein the at least one environmental medium treatment agent is pH dependent.

83. The composition of claim 1, wherein the at least one environmental medium treatment agent includes at least one time-release formulation.

84. The composition of claim 1, wherein the at least one environmental medium treatment agent is provided by at least one of synthesizing the at least one environmental medium treatment agent, expressing the at least one environmental medium treatment agent on the microorganism surface, or extracellular secretion of the at least one environmental medium treatment agent.

85. The composition of claim 1, wherein the at least one environmental medium treatment agent is encoded by at least one vector.

105. The composition of claim 1, further comprising at least one detection material associated with the at least one modified microorganism.

106. The composition of claim 105, wherein the at least one detection material includes at least one, sensor, contrast agent, or electronic identification device.

107. The composition of claim 106, wherein the at least one sensor includes at least one biosensor.

108. The composition of claim 106, wherein the at least one electronic identification device includes at least one radio frequency identification device.

109. The composition of claim 105, wherein the at least one detection material includes at least one of a radioactive, luminescent, colorimetric or odorous substance.

110. The composition of claim 105, wherein the detection material includes at least one of a diamagnetic particle, ferromagnetic particle, paramagnetic particle, super paramagnetic particle, particle with altered isotope, or other magnetic particle.

111. The composition of claim 105, wherein the detection material includes at least one of a DNA or RNA device.

112. The composition of claim 105, wherein the detection material or a prectirsOr thereof is encoded by the at least one heterologous genetic element encoding at least one environmental medium treatment agent.

113. The composition of claim 105, wherein the detection material includes the at least one environmental medium treatment agent or a metabolite thereof.

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114. A method of administering at least one environmental medium treatment agent to at least one environmental medium, comprising:

providing at least one composition to at least one environmental medium;

wherein the at least one composition includes at least one modified microorganism including at least one heterologous genetic element encoding at least one environmental medium treatment agent, and at least one genetic element inducible to initiate death of the at least one modified microorganism.

117. The method of claim 114, further comprising administering at least one of an inducer or repressor of the heterologous genetic element encoding at least one environmental medium treatment agent.

118. The method of claim 114, further comprising administering at least one of an inducer or repressor of the at least one genetic element inducible to initiate death of the at least one modified microorganism.

119. The method of claim 114, wherein the composition provides an effective amount of at least one environmental medium treatment agent in relation to at least one intended outcome.

120. The method of claim 114, wherein the composition provides an effective amount of at least one environmental medium treatment agent in relation to establishment or re-establishment of organisms in the at least one environmental medium.

121. The method of claim 114, wherein the composition provides an effective amount of at least one environmental medium treatment agent in relation to revitalization of the at least one environmental medium associated with at least one of fire;

flood, contamination, drought, deforestation, temperature change, or habitat change.

122. The method of claim 114, wherein the at least one environmental medium includes at least one of water, soil, food products, or air or other gas.

123. The method of claim 114, wherein the at least one environmental medium includes at least one of ground water, surface water, effluent, or wastewater.

124. The method of claim 114, wherein the at least one environmental medium includes at least one of a lake, river, stream, sludge, slurry, sediment, sewage, ocean, fountain, or other water.

127. The method of claim 114, wherein the at least one modified microorganism includes at least one obligate microorganism.

129. The method of claim 114, wherein the at least one composition further comprises at least one metabolite utilizable by the at least one modified microorganism.

140. The method of claim 114, further comprising detecting at least one of the presence, amount, concentration, or location of the at least one modified microorganism, or the environmental medium treatment agent.

141. The method of claim 114, further comprising selecting for administration an amount or type of composition.

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