Compositions and methods for therapeutic delivery with microorganisms

• US 20110183351A1
• Filed: 01/22/2010
• Published: 07/28/2011
• Est. Priority Date: 01/22/2010
• Status: Abandoned Application

• Current Assignee: Searete LLC (Intellectual Ventures LLC)
• Original Assignee: Searete LLC (Intellectual Ventures LLC)
• Inventors: Rinaldo, John D. JR., Lord, Robert W., Jung, Edward K.Y., Levien, Royce A., Wood, Lowell L. JR., Hyde, Roderick A., Malamud, Mark A.
• Application Number:
  US12/657,605
• Publication Number:
  US 20110183351A1
• Time in Patent Office: Days
• US Class Current: 435/7.1
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 35/74 Bacteria therapeutic use of a bacterial protein A61K38/00
• CPC Class Codes: A61K 35/747 Lactobacilli, e.g. L. acidophilus or L. brevis
• CPC Class Codes: A61K 36/064 Saccharomycetales, e.g. baker's yeast
• CPC Class Codes: A61K 38/162 from virus
• CPC Class Codes: A61K 38/1841 Transforming growth factor [TGF]
• CPC Class Codes: A61K 38/2013 IL-2
• CPC Class Codes: A61K 38/2066 IL-10
• CPC Class Codes: A61K 38/208 IL-12
• CPC Class Codes: C12N 1/36 Adaptation or attenuation of cells
• CPC Class Codes: C12N 15/635 Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

Certain embodiments disclosed relate to compositions, including therapeutic compositions, methods, devices, and systems that include modified microorganisms including at least one genetic element encoding at least one therapeutic agent or environmental treatment agent.

Claims

1. A system comprising:

at least one computing device;

at least one delivery device configured to retain and dispense at least one composition to at least one environmental medium; and

a recordable medium including one or more instructions that when executed on a computing device cause the computing device to regulate dispensing of at least a portion of the at least one composition,

wherein the at least one composition includes at least one modified microorganism including at least one heterologous genetic element encoding at least one environmental medium treatment agent; and

at least one genetic element inducible to initiate death of the at least one modified microorganism.

2. The system of claim 1, wherein the at least one computing device includes at least one computing device located on or in the at least one delivery device.

3. The system of claim 1, wherein the at least one computing device includes at least one computing device located remotely from the at least one delivery device.

4. The system of claim 1, wherein the at least one computing device includes one or more of a desktop computer, workstation computer, or computing system.

5. The system of claim 1, wherein the at least one computing system includes one or more of a cluster of processors, a networked computer, a tablet personal computer, a laptop computer, a mobile device, a mobile telephone, or a personal digital assistant computer.

6. The system of claim 1, further comprising one or more instructions that when executed on the at least one computing device cause the at least one computing device to generate at least one output to a user.

7. The system of claim 6, wherein the at least one output includes at least one graphical illustration of one or more of the at least one composition, at least one constituent thereof, or at least one product thereof;

at least one cell or substance associated with the at least one environmental medium;

or the at least one property of dispensing the at least one delivery device.

10. The system of claim 6, wherein the at least one output includes at least one protocol for administering the at least one composition to the at least one environmental medium.

11. The system of claim 6, wherein the user includes at least one entity.

12. The system of claim 11, wherein the entity includes at least one person, or computer.

13. The system of claim 6, wherein the output includes an output to a user readable display.

14. The system of claim 13, wherein the user readable display includes a human readable display.

15. The system of claim 13, wherein the user readable display includes one or more active displays.

16. The system of claim 13, wherein the user readable display includes one or more passive displays.

17. The system of claim 13, wherein the user readable display includes one or more of a numeric format, graphical format, or audio format.

18. The system of claim 1, wherein the at least one environmental medium includes at least one of water, soil, food product, or air or other gas.

19. The system of claim 1, wherein the at least one environmental medium includes at least one of ground water, surface water, effluent, or wastewater.

20. The system of claim 19, wherein the water includes at least one of a lake, river, stream, sewage, sludge, slurry, sediment, ocean, fountain, or other water.

21. The system of claim 1, wherein the at least one environmental medium includes at least one of metal, concrete, cement, textiles, fabric, wood, mineral ore, or rock.

22. The system of claim 1, wherein the at least one environmental medium treatment agent includes at least one plant hormone.

23. The system of claim 22, wherein the at least one plant hormone includes at least one of an auxin, abscisic acid, cytokinin, ethylene, gibberellin, brassinolide, salicyclic acid, jasmonate, polyamine, plant peptide hormone, nitric oxide, strigolactone, or other compound.

24. The system of claim 1, further comprising one or more instructions for obtaining genetic sequence information from at least one microorganism isolated from the at least one environmental medium.

25. The system of claim 24, further comprising one or more instructions for modifying the at least one microorganism isolated from the at least one environmental medium.

28. The system of claim 1, further comprising one or more instructions for predetermining at least one microorganism type for modifying to produce at least one environmental medium treatment agent based on at least one feature of the at least one environmental medium.

29. The system of claim 28, wherein the at least one feature of the at least one environmental medium includes at least one property of one or more microorganism populations associated with the at least one environmental medium.

33. The system of claim 1, further comprising one or more instructions for selecting the composition, or a constituent thereof.

34. The system of claim 1, further comprising one or more instructions for administering the at least one composition or a constituent thereof to at least one environmental medium.

8-9. -9. (canceled)

26-27. -27. (canceled)

30-32. -32. (canceled)

35. A computer-implemented method comprising:

one or more instructions for regulating dispensing of at least one composition from at least one delivery device to at least one environmental medium, the at least one composition including at least one modified microorganism including at least one heterologous genetic element encoding at least one environmental medium treatment agent; and

at least one genetic element inducible to initiate death of the at least one modified microorganism.

36. The computer-implemented method of claim 35, further comprising generating at least one output to a user.

37. The computer-implemented method of claim 36, wherein the at least one output includes at least one graphical illustration of one or more of the at least one composition, at least one constituent thereof, or at least one product thereof;

at least one metabolite utilizable by the at least one modified microorganism;

at least one property of the at least one delivery device;

or at least one property of dispensing the at least one delivery device.

40. The computer-implemented method of claim 36, wherein the at least one output includes at least one protocol for administering the at least one composition to the at least one environmental medium.

41. The computer-implemented method of claim 36, wherein the user includes at least one entity.

42. The computer-implemented method of claim 41, wherein the entity includes at least one person, or computer.

43. The computer-implemented method of claim 36, wherein the output includes an output to a user readable display.

44. The computer-implemented method of claim 43, wherein the user readable display includes a human readable display.

45. The computer-implemented method of claim 43, wherein the user readable display includes one or more active displays.

46. The computer-implemented method of claim 43, wherein the user readable display includes one or more passive displays.

47. The computer-implemented method of claim 43, wherein the user readable display includes one or more of a numeric format, graphical format, or audio format.

49. The computer-implemented method of claim 35, further comprising one or more instructions to dispense at least a portion of the at least one composition or a constituent thereof, to at least one environmental medium.

50. The computer-implemented method of claim 35, further comprising one or more instructions for dispensing at least a portion of at least one inducer formulated to induce the at least one genetic element inducible to initiate death of the at least one modified microorganism.

51. The computer-implemented method of claim 35, further comprising receiving information related to one or more environmental medium indicators prior to, during, or subsequent to administering the at least one composition or a constituent thereof, to the at least one environmental medium.

52. The computer-implemented method of claim 51, wherein the receiving information related to one or more environmental medium indicators includes information from at least one of an assay, image, or gross assessment of the at least one environmental medium prior to, during, or subsequent to administering the at least one composition.

53. The computer-implemented method of claim 52, wherein the assay includes at least one technique including spectroscopy, microscopy, electrochemical detection, polynucleotide detection, histological examination, biopsy analysis, fluorescence resonance energy transfer, electron transfer, enzyme assay, electrical conductivity, isoelectric focusing, chromatography, immunoprecipitation, immunoseparation, aptamer binding, filtration, electrophoresis, immunoassay, or radioactive assay.

54. The computer-implemented method of claim 52, wherein the at least one image includes one or more images acquired by at least one of laser, holography, x-ray crystallography, optical coherence tomography, computer-assisted tomography scan, computed tomography, magnetic resonance imaging, positron-emission tomography scan, ultrasound, x-ray, electrical-impedance monitoring, microscopy, spectrometry, flow cytommetry, radioisotope imaging, thermal imaging, infrared visualization, multiphoton calcium-imaging, photography, or in silico generation.

55. The computer-implemented method of claim 35, further comprising one or more instructions for dispensing at least a portion of the at least one composition or constituent thereof, to the at least one environmental medium in response to the one or more environmental medium indicators.

56. The computer-implemented method of claim 35, further comprising one or more instructions for dispensing at least a portion of at least one inducer formulated to induce the at least one genetic element inducible to initiate death of the at least one modified microorganism, to the at least one environmental medium in response to the one or more biological tissue indicators.

57. The computer-implemented method of claim 56, wherein the one or more environmental medium indicators relate to one or more of:

administration of the at least one composition, at least one constituent thereof, or at least one product thereof;

administration of at least one metabolite utilizable by the at least one modified microorganism;

administration of the at least one modified microorganism;

status of the at least one modified microorganism of the at least one composition;

status of the at least one composition;

status of the at least one environmental medium treatment agent;

or status of at least one metabolite utilizable by the at least one modified microorganism;

or depletion of at least one metabolite utilizable by the at least one modified microorganism.

58. The computer-implemented method of claim 35, further comprising obtaining genetic sequence information from at least one microorganism isolated from the at least one environmental medium.

59. The computer-implemented method of claim 58, further comprising one or more instructions for modifying the at least one microorganism isolated from the at least one environmental medium.

60. The computer-implemented method of claim 59, further comprising one or more instructions for amplifying the at least one microorganism isolated from the at least one environmental medium.

61. The computer-implemented method of claim 59, further comprising one or more instructions for reinstating the at least one microorganism isolated from the at least one environmental medium subsequent to modification.

38-39. -39. (canceled)

48. (canceled)

62-80. -80. (canceled)