FLUORESCENT LABELING AGENT AND FLUORESCENT LABELING METHOD

• US 20110189101A1
• Filed: 11/29/2007
• Published: 08/04/2011
• Est. Priority Date: 12/01/2006
• Status: Abandoned Application

• Current Assignee: National University Corporation Shimane University
• Original Assignee: National University Corporation Shimane University
• Inventors: Fujita, Yasuhisa, Nakamura, Morihiko
• Application Number:
  US12/312,876
• Publication Number:
  US 20110189101A1
• Time in Patent Office: Days
• US Class Current: 424/9.6
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C01P 2002/72 by d-values or two theta-values, e.g. as X-ray diagram
• CPC Class Codes: C01P 2002/84 by UV- or VIS- data
• CPC Class Codes: C01P 2004/04 obtained by TEM, STEM, STM or AFM
• CPC Class Codes: C01P 2004/64 Nanometer sized, i.e. from 1-100 nanometer
• CPC Class Codes: C01P 2004/84 one phase coated with the other
• CPC Class Codes: C09C 1/02 Compounds of alkaline earth metals or magnesium C09C1/0003, C09C1/0009, C09C1/0015, C09C1/0078 take precedence; dolomitic solids C09C1/009
• CPC Class Codes: C09C 1/04 Compounds of zinc C09C1/0003, C09C1/0009, C09C1/0015, C09C1/0078 take precedence
• CPC Class Codes: C09C 1/043 Zinc oxide
• CPC Class Codes: C09K 11/54 containing zinc or cadmium
• CPC Class Codes: C09K 11/565 with zinc cadmium
• CPC Class Codes: C09K 11/574 with zinc or cadmium
• CPC Class Codes: C09K 11/59 containing silicon
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: G01N 33/587 Nanoparticles

Abstract

Disclosed is use of zinc oxide nanoparticles as an exciton emission source in vivo or in vitro. Also disclosed is a fluorescent labeling agent comprising a zinc oxide nanoparticle and a substance capable of selectively binding to a target in vivo or in vitro, wherein the substance is bound to the zinc oxide nanoparticle via at least one binder. Further disclosed is a fluorescent labeling agent comprising a zinc oxide nanoparticle of which the surface is coated with a coating film selected from ZnS, Mg_xZn_1-xO (where 0<x≦1) and SiO₂, and a substance capable of selectively binding to a target in vivo or in vitro, wherein the substance is bound to the zinc oxide nanoparticle via at least one binder.

Claims

1. Use of zinc oxide nanoparticles as an exciton emission source in vivo or in vitro.

2. A fluorescent labeling agent comprising zinc oxide nanoparticles for use in vivo or in vitro, wherein the exciton emission of the zinc oxide nanoparticles is utilized.

3. The fluorescent labeling agent as claimed in claim 2, wherein a substance capable of selectively binding to a target in vivo or in vitro is bound to the zinc oxide nanoparticle via at least one binder.

4. The fluorescent labeling agent as claimed in claim 2, wherein a surface of the zinc oxide nanoparticle is coated with a coating film selected from ZnS, Mg_xZn_1-xO (where 0<

x≦

1) and SiO₂, and a substance capable of selectively binding to a target in vivo or in vitro is bound to the zinc oxide nanoparticle via at least one binder.

5. The fluorescent labeling agent as claimed in claim 3, wherein the binder is a substance that modifies an amino group or a carboxyl group as a functional group on the particle side.

6. The fluorescent labeling agent as claimed in claim 3, wherein the binder is AEDP (3-[(2-aminoethyl)dithio]propionic acid), ASBA (4-(p-azidosalicylamido)butylamine), EDC (1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride), or sulfo-NHS (N-hydroxysulfosuccinimide)-added EDC, which binds to the substance capable of selectively binding to a target.

7. The fluorescent labeling agent as claimed in claim 3, wherein the substance capable of selectively binding to a target is an antibody, an enzyme, a lectin or a nucleic acid.

8. The fluorescent labeling agent as claimed in claim 3, wherein the target is a tumor cell, a leukemia cell, a virus-infected cell, or a normal cell, a protein, an enzyme or a nucleic acid.

12. The fluorescent labeling agent as claimed in claim 4, wherein the binder is a substance that modifies an amino group or a carboxyl group as a functional group on the particle side.

13. The fluorescent labeling agent as claimed in claim 4, wherein the binder is AEDP (3-[(2-aminoethyl)dithio]propionic acid), ASBA (4-(p-azidosalicylamido)butylamine), EDC (1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride), or sulfo-NHS (N-hydroxysulfosuccinimide)-added EDC, which binds to the substance capable of selectively binding to a target.

14. The fluorescent labeling agent as claimed in claim 4, wherein the substance capable of selectively binding to a target is an antibody, an enzyme, a lectin or a nucleic acid.

15. The fluorescent labeling agent as claimed in claim 4, wherein the target is a tumor cell, a leukemia cell, a virus-infected cell, or a normal cell, a protein, an enzyme or a nucleic acid.

9. A fluorescent labeling method, wherein the exciton emission of zinc oxide nanoparticles is utilized in vivo or in vitro.

10. A fluorescent labeling method, wherein a substance capable of selectively binding to a target in vivo or in vitro is bound to a zinc oxide nanoparticle via at least one binder, and this is bound to a target, and then irradiated with UV rays as excitation light to thereby make the target part shine by the exciton emission of zinc oxide.

11. A fluorescent labeling method, wherein the surface of a zinc oxide nanoparticle is coated with a coating film selected from ZnS, Mg_xZn_1-xO (where 0<

x≦

1) and SiO₂, the nanoparticle is bound to a substance capable of selectively binding to a target in vivo or in vitro, via at least one binder, and this is bound to a target, and then irradiated with UV rays as excitation light to thereby make the target part shine by the exciton emission of zinc oxide.