METHOD FOR GENERATION AND REGULATION OF IPS CELLS AND COMPOSITIONS THEREOF
First Claim
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1. A method of generating an induced pluripotent stem (iPS) cell comprising:
- a) contacting a somatic cell with a nuclear reprogramming factor; and
b) contacting the cell of (a) with a microRNA that alters RNA levels or activity within the cell, thereby generating an iPS cell.
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Abstract
The present invention provides methods for generating induced pluripotent stem (iPS) cells having an increased efficiency of induction as compared with conventional methods. The method includes treating a somatic cell with a nuclear reprogramming factor in combination with an agent that alters microRNA levels or activity in the cell and/or a p21 inhibitor. The invention further provides iPS cells generated by such methods, as well as clinical and research uses for such iPS cells.
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Citations
29 Claims
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1. A method of generating an induced pluripotent stem (iPS) cell comprising:
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a) contacting a somatic cell with a nuclear reprogramming factor; and b) contacting the cell of (a) with a microRNA that alters RNA levels or activity within the cell, thereby generating an iPS cell. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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- 20. The use of microRNA for increasing efficiency of generating of iPS cells.
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22. A combination of miR sequences selected from the group consisting of an least two or more of miR-17, miR-25, miR-93, miR-106a, miR-106b, miR-21, miR-29a, miR-302b cluster, miR let-7 family member, or a combination thereof.
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23. A method of generating an induced pluripotent stem (iPS) cell comprising:
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a) contacting a somatic cell with a nuclear reprogramming factor; and b) contacting the cell of (a) with an inhibitor of microRNA, thereby generating an iPS cell. - View Dependent Claims (24, 25, 26, 27, 28, 29)
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Specification