KIT AND METHOD FOR EVALUATING DETECTION PROPERTIES IN AMPLIFICATION REACTIONS

• US 20110189763A1
• Filed: 07/24/2009
• Published: 08/04/2011
• Est. Priority Date: 07/25/2008
• Status: Abandoned Application

• Current Assignee: Qiagen GmbH (Qiagen NV)
• Original Assignee: Biotype GmbH
• Inventors: Brabetz, Werner, Gabert, Jorg
• Application Number:
  US13/055,597
• Publication Number:
  US 20110189763A1
• Time in Patent Office: Days
• US Class Current: 435/287.2
• CPC Class Codes: C12Q 1/6851 Quantitative amplification
• CPC Class Codes: C12Q 2545/113 with an external standard/control, i.e. control reaction is separated from the test/target reaction

Abstract

The invention relates to a kit comprising, (a) a first group of containers, comprising two or more containers with human genomic DNA, wherein the human genomic DNA in each of the two or more containers is of different human origin and the amount of DNA in the container is defined, (b) a second group of containers, comprising two or more containers with human genomic DNA, wherein the concentration of the DNA varies between the containers and the concentration of the DNA in the container is defined and (c) a third group of containers, comprising two or more containers with human genomic DNA, wherein the human genomic DNA in each container is a mixture of genomic DNA and is from at least two different individuals. The invention also relates to an evaluation tool and methods of use of the kit.

Claims

1. Kit comprising,

a. a first group of containers, comprising two or more containers with human genomic DNA, wherein the human genomic DNA in each of the two or more containers is of different human origin and the amount of DNA in the container is defined;

b. a second group of containers, comprising two or more containers with human genomic DNA, wherein the concentration of the DNA varies between the containers and the concentration of the DNA in the container is defined;

c. a third group of containers, comprising two or more containers with human genomic DNA, wherein the human genomic DNA in each container is a mixture of genomic DNA and is from at least two different individuals.

2. Kit according to claim 1, wherein the first group of containers comprises two or more containers each comprising human genomic DNA, wherein at least one DNA is from a male individual and one DNA is from a female individual.

3. Kit according to claim 1, wherein the human genomic DNA in the second group of containers has a quantity of between 5 pg and 10 ng.

4. Kit according to claim 1, wherein the mixture of genomic DNAs in the third group of containers is has a ratio of between 1 to 100 and 100 to 1, wherein the larger fraction has a quantity of between 50 pg and 2 ng.

5. Kit according to claim 1, wherein the mixture of genomic DNAs in the third group of containers is has a ratio of between 1 to 50 and 50 to 1, wherein the larger fraction has a quantity of between 200 pg and 800 pg.

6. Kit according to claim 1, wherein the third group of containers comprises at least one container which carries an equal amount of said two genomic DNAs.

7. Kit according to claim 1, wherein the kit comprises a fourth group of containers and this group comprises containers with human genomic DNA and an amplification reaction inhibitor.

8. Kit according to claim 7, wherein the amplification reaction inhibitor is selected from the group comprising humic acid, hemin (chloro [3,7,12,17-tetramethyl-8,13-divinylporphyrin-2,18-dipropanoato(2-)] iron(III)), indigo and bile salts (equimolar mixture of sodium salts of cholic acid and desoxycholic acid).

9. Kit according to claim 7, wherein the fourth group of containers comprises at least one container with human genomic DNA from a male individual and one container with human genomic DNA from a female individual.

10. Kit according to claim 7, wherein the kit additionally comprises a fifth group of containers comprising at least one container with a first non-human genomic DNA sample.

11. Kit according to claim 10, wherein the non-human genomic DNA sample is selected from the group of dog, cat, bovine, horse, sheep, chicken, gorilla, orangutan, chimpanzee, macaque, E. coli, and Candida albicans.

12. Kit according to claim 10, wherein the kit additionally comprises a sixth group of containers comprising at least one container with a first human genomic DNA sample which is degraded.

13. Kit according to claim 12, wherein the sixth group comprises more than one container, wherein each container comprises a human genomic DNA sample and wherein each genomic DNA sample has a different state of degradation.

14. Kit according to claim 12, wherein the kit additionally comprises a seventh group of containers comprising at least one container with a first human genomic DNA sample as well as a second human genomic DNA sample, wherein the second human genomic DNA is from a different individual than the first sample and the second sample is degraded.

15. Kit according to claim 14, wherein the kit additionally comprises an eighth group of containers comprising at least one container with no DNA but either water or a buffer or another solution which may be added to an amplification reaction.

16. Kit according to claim 1, wherein the containers are wells in a microtiter plate and the plate has 6, 24, 96, 384 or even 1536 sample wells arranged in a 2:

3 rectangular matrix.