METHODS OF PREPARING FEEDER CELLS-FREE, XENO-FREE HUMAN EMBRYONIC STEM CELLS AND STEM CELL CULTURES PREPARED USING SAME
First Claim
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1. A culture medium which comprises transforming growth factor β
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1 (TGFβ
1) and basic fibroblast growth factor (bFGF), said culture medium being capable of maintaining pluripotent stem cell in an undifferentiated state.
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Abstract
The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.
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Citations
20 Claims
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1. A culture medium which comprises transforming growth factor β
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1 (TGFβ
1) and basic fibroblast growth factor (bFGF), said culture medium being capable of maintaining pluripotent stem cell in an undifferentiated state. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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1 (TGFβ
Specification