IN SITU ANALYSIS OF TISSUES
First Claim
1. A method for analyzing protein content in a tissue comprising:
- (a) providing an intact tissue comprising a first spatially discrete microregion;
(b) subjecting said microregion to one or more physical or chemical treatments; and
(c) analyzing a protein sample from said microregion, thereby providing analysis of protein content in said tissue.
0 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides for the simultaneous assessment of a plurality of tissue regions or microregions, the benefit being homogeneity of the sampling, both in terms of tissue content and timing. Discrete regions of a tissue sample, such as those demarcated by microwells formed within the tissue itself or tissue plugs removed from the tissue in a spatially referenced fashion, can be treated with one or more physical or chemical treatments to liberate target molecules of interest. Subsequent analysis of said target molecules by, e.g., mass spectroscopy, permits identification of a variety of biological parameters, including those associated with disease or therapy.
-
Citations
60 Claims
-
1. A method for analyzing protein content in a tissue comprising:
-
(a) providing an intact tissue comprising a first spatially discrete microregion; (b) subjecting said microregion to one or more physical or chemical treatments; and (c) analyzing a protein sample from said microregion, thereby providing analysis of protein content in said tissue. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40)
-
-
41. A method for analyzing nucleic acid content in a tissue comprising:
-
(a) providing an intact tissue comprising a first spatially discrete microregion; (b) subjecting said microregion to one or more physical or chemical treatments; and (c) analyzing a nucleic acid sample from said microregion, thereby providing analysis of nucleic acid content in said tissue.
-
-
42. A method for analyzing lipid content in a tissue comprising:
-
(a) providing an intact tissue comprising a first spatially discrete microregion; (b) subjecting said microregion to one or more physical or chemical treatments; and (c) analyzing a lipid sample from said microregion, thereby providing analysis of lipid content in said tissue.
-
-
43. A method for analyzing carbohydrate content in a tissue comprising:
-
(a) providing an intact tissue comprising a first spatially discrete microregion; (b) subjecting said microregion to one or more physical or chemical treatments; and (c) analyzing a carbohydrate sample from said microregion, thereby providing analysis of carbohydrate content in said tissue.
-
-
44. A method for analyzing the delivery of an exogenous agent to a tissue comprising:
-
(a) providing an intact tissue comprising a first spatially discrete microregion; (b) contacting said tissue with said exogenous agent; (c) subjecting at said first spatially discrete microregion to one or more physical or chemical treatments; and (d) analyzing a sample from said first spatially discrete microregion, thereby providing analysis of the delivery of said exogenous agent to said tissue. - View Dependent Claims (45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58)
-
-
59. A method for analyzing an endogenous metabolite in a tissue comprising:
-
(a) providing an intact tissue comprising a first spatially discrete microregion; (b) subjecting said microregion to one or more physical or chemical treatments; and (c) analyzing a endogenous metabolite sample from said microregion, thereby providing analysis of endogenous metabolite content in said tissue. - View Dependent Claims (60)
-
Specification