COMPOSITIONS FOR USE IN IDENTIFICATION OF ANTIBIOTIC-RESISTANT BACTERIA
First Claim
1. A purified oligonucleotide primer pair for identifying an antibiotic-resistant bacterium in a sample, said primer pair comprising a forward primer and a reverse primer, each configured to hybridize to nucleic acid of two or more different species or strains of bacteria in a nucleic acid amplification reaction which produces an amplification product between about 29 to about 200 nucleobases in length, said amplification product comprising portions corresponding to a forward primer hybridization region, a reverse primer hybridization region and an intervening region having a base composition which varies among amplification products produced from nucleic acid of said two or more different species or strains of bacteria, said base composition of said intervening region providing a means for identifying said antibiotic-resistant bacterium.
1 Assignment
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Accused Products
Abstract
The present invention relates generally to identification of antibiotic-resistant bacteria and provides methods, compositions and kits useful for this purpose when combined, for example, with molecular mass or base composition analysis.
101 Citations
48 Claims
- 1. A purified oligonucleotide primer pair for identifying an antibiotic-resistant bacterium in a sample, said primer pair comprising a forward primer and a reverse primer, each configured to hybridize to nucleic acid of two or more different species or strains of bacteria in a nucleic acid amplification reaction which produces an amplification product between about 29 to about 200 nucleobases in length, said amplification product comprising portions corresponding to a forward primer hybridization region, a reverse primer hybridization region and an intervening region having a base composition which varies among amplification products produced from nucleic acid of said two or more different species or strains of bacteria, said base composition of said intervening region providing a means for identifying said antibiotic-resistant bacterium.
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24. An isolated amplification product for identification of an antibiotic-resistant bacterium, said amplification product produced by a process comprising:
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a) amplifying nucleic acid of a bacterium in a reaction mixture comprising a primer pair, said primer pair comprising a forward primer and a reverse primer, each configured to hybridize to nucleic acid of two or more different species or strains of bacteria in a nucleic acid amplification reaction, said amplification product having a length of about 29 to about 200 nucleobases and comprising portions corresponding to a forward primer hybridization region, a reverse primer hybridization region and an intervening region having a base composition which varies among amplification products produced from nucleic acid of said two or more different species or strains of bacteria, said base composition of said intervening region providing a means for identifying said antibiotic-resistant bacterium; and b) isolating said amplification product from said reaction mixture. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 47, 48)
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44. A kit comprising one or more purified primer pairs for identifying an antibiotic-resistant bacterium in a sample, each member of said one or more primer pairs having at least 70% sequence identity with a corresponding member of one or more primer pairs selected from the group consisting of:
- SEQ ID NOs;
16;
2, 5;
18, 10;
17, 14;
19, 12;
6, 9;
3, 15;
7, 21;
7, 1;
4, 11;
13, 8;
20, 27;
24, 22;
25, and 26;
23. - View Dependent Claims (45, 46)
- SEQ ID NOs;
Specification