Renewable Chemicals and Fuels From Oleaginous Yeast
First Claim
1. A method of producing a fuel or a chemical, the method comprising the steps of:
- (a) heterotrophically culturing an oleaginous yeast in the presence of a fixed carbon source, wherein;
(i) the fixed carbon source is selected from the group consisting of glycerol, depolymerized cellulosic material, sucrose, molasses, glucose, arabinose, galactose, xylose, fructose, mannose, and acetate; and
(ii) the oleaginous yeast accumulates at least 10% of its dry cell weight as triacylglyceride;
(b) isolating the triacylglyceride from the oleaginous yeast; and
(c) subjecting the isolated triacylglyceride to a chemical reaction selected from the group consisting of cracking, (hydro)deoxygenation, esterification, and isomerization, and optionally, distillation.
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Accused Products
Abstract
The invention provides methods of manufacturing alkanes from triglyceride oils produced through fermentation of oil-bearing microbes. The processes provided herein can utilize a variety of carbohydrate feedstocks including cane bagasse, sugar beet pulp, corn stover, glycerol, corn starch, sorghum, molasses, waste glycerol, and other renewable materials. These processes further comprise hydrotreating, hydrocracking, isomerization, distillation, and other petrochemical processes for use with oil-bearing microbes and products derived therefrom to manufacture fuels. Particular embodiments include the manufacture of ASTM D975 and ASTM D1655 compliant fuels. Genetically engineered microbes provided herein can be used in the manufacture of renewable diesel and renewable jet fuel.
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Citations
27 Claims
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1. A method of producing a fuel or a chemical, the method comprising the steps of:
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(a) heterotrophically culturing an oleaginous yeast in the presence of a fixed carbon source, wherein; (i) the fixed carbon source is selected from the group consisting of glycerol, depolymerized cellulosic material, sucrose, molasses, glucose, arabinose, galactose, xylose, fructose, mannose, and acetate; and (ii) the oleaginous yeast accumulates at least 10% of its dry cell weight as triacylglyceride; (b) isolating the triacylglyceride from the oleaginous yeast; and (c) subjecting the isolated triacylglyceride to a chemical reaction selected from the group consisting of cracking, (hydro)deoxygenation, esterification, and isomerization, and optionally, distillation. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A method for producing a triacylglyceride, said method comprising the steps of culturing an oleaginous yeast in a culture media to provide an oleaginous yeast comprising at least about 10% triacylglyceride by dry weight;
- and extracting the triacylglyceride from the oleaginous yeast.
- View Dependent Claims (13, 14, 15, 16)
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17. A fuel or chemical produced by the method comprising the steps of:
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(a) heterotrophically culturing an oleaginous yeast in the presence of a fixed carbon source, wherein; (i) the fixed carbon source is selected from the group consisting of glycerol, depolymerized cellulosic material, sucrose, molasses, glucose, arabinose, galactose, xylose, fructose, mannose and acetate; and (ii) the oleaginous yeast accumulates at least 10% of its dry cell weight as triacylglyceride; (b) isolating the triacylglyceride from the oleaginous yeast; and (c) subjecting the isolated triacylglyceride to a chemical reaction selected from the group consisting of cracking, (hydro)deoxygenation, esterification, and isomerization, and optionally, distillation. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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Specification