Systems and methods for manipulating a molecule in a nanopore
First Claim
1. A method of manipulating a molecule in a nanopore embedded in a lipid bilayer, including:
- applying an acquiring electrical stimulus level across a lipid bilayer wherein a region of the lipid bilayer containing the nanopore is characterized by a resistance and wherein the acquiring electrical stimulus level tends to draw the molecule from a surrounding fluid into the nanopore;
detecting a change in the resistance of the lipid bilayer resulting from the acquisition of at least a portion of a molecule into the nanopore;
changing the acquiring electrical stimulus level to a holding electrical stimulus level wherein the portion of the molecule remains in the nanopore upon the changing of the acquiring electrical stimulus level to the holding electrical stimulus level.
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Accused Products
Abstract
Techniques for manipulating a molecule in a nanopore embedded in a lipid bilayer are described. In one example, an acquiring electrical stimulus level is applied across a lipid bilayer wherein a region of the lipid bilayer containing the nanopore is characterized by a resistance and wherein the acquiring electrical stimulus level tends to draw the molecule from a surrounding fluid into the nanopore, a change in the resistance of the lipid bilayer resulting from the acquisition of at least a portion of a molecule into the nanopore is detected, the acquiring electrical stimulus level is changed to a holding electrical stimulus level wherein the portion of the molecule remains in the nanopore upon the changing of the acquiring electrical stimulus level to the holding electrical stimulus level.
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Citations
72 Claims
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1. A method of manipulating a molecule in a nanopore embedded in a lipid bilayer, including:
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applying an acquiring electrical stimulus level across a lipid bilayer wherein a region of the lipid bilayer containing the nanopore is characterized by a resistance and wherein the acquiring electrical stimulus level tends to draw the molecule from a surrounding fluid into the nanopore; detecting a change in the resistance of the lipid bilayer resulting from the acquisition of at least a portion of a molecule into the nanopore; changing the acquiring electrical stimulus level to a holding electrical stimulus level wherein the portion of the molecule remains in the nanopore upon the changing of the acquiring electrical stimulus level to the holding electrical stimulus level. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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25. A system for manipulating a molecule in a nanopore embedded in a lipid bilayer, including:
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a variable voltage source configured to apply an acquiring electrical stimulus level across a lipid bilayer wherein a region of the lipid bilayer containing the nanopore is characterized by a resistance and wherein the acquiring electrical stimulus level tends to draw the molecule from a surrounding fluid into the nanopore; a sensing circuit configured to detect a change in the resistance of the lipid bilayer resulting from the acquisition of at least a portion of a molecule into the nanopore; wherein the variable voltage source is further configured to change the acquiring electrical stimulus level to a holding electrical stimulus level upon the changing of the acquiring electrical stimulus level to the holding electrical stimulus level wherein the portion of the molecule remains in the nanopore. - View Dependent Claims (26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48)
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49. A system for manipulating a molecule in a nanopore embedded in a lipid bilayer, to including:
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means for applying an acquiring electrical stimulus level across a lipid bilayer wherein a region of the lipid bilayer containing the nanopore is characterized by a resistance and wherein the acquiring electrical stimulus level tends to draw molecule from a surrounding fluid into the nanopore; means for detecting a change in the resistance of the lipid bilayer resulting from the acquisition of at least a portion of a molecule into the nanopore; means for changing the acquiring electrical stimulus level to a holding electrical stimulus level wherein the portion of the molecule remains in the nanopore upon the changing of the acquiring electrical stimulus level to the holding electrical stimulus level. - View Dependent Claims (50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72)
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Specification