Method and Markers for Determining the Genotype of Horned/Polled Cattle
First Claim
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1. A method for identifying the polled phenotype in a bovine subject, comprising detecting a dominant polled haplotype allele in a nucleic acid sample from the subject selected from:
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Abstract
Provided herein are methods to discover and use single nucleotide polymorphisms (SNP) for determining the genotype of a horned/polled ruminant subject. The present invention further provides specific nucleic acid sequences, SNPs, and SNP patterns that can be used for determining the genotype of a horned/polled ruminant subject.
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20 Claims
- 1. A method for identifying the polled phenotype in a bovine subject, comprising detecting a dominant polled haplotype allele in a nucleic acid sample from the subject selected from:
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6. A method for identifying the horned phenotype in a bovine subject, comprising:
detecting a pair of recessive horned haplotype alleles in a nucleic acid sample from the subject, wherein each haplotype allele is independently selected from; - View Dependent Claims (7, 8, 9)
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10. A method for identifying the polled phenotype in a bovine subject:
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a. detecting a GATCGG haplotype allele in a nucleic acid sample from the subject, wherein the sequence of letters of the haplotype allele represent the nucleotide occurrence of a single nucleotide polymorphism corresponding to nucleotide position 300 of SEQ ID NOs;
53 (marker MMBT25287), 59 (marker MMBT25303), 52 (marker MMBT25281), 63 (marker MMBT25316), 62 (marker MMBT25314), and 61 (marker MMBT25313) respectively; andb. detecting the GATCGG haplotype in a nucleic acid sample from at least one polled parent of the subject. - View Dependent Claims (11, 12)
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16. A method for identifying a haplotype allele associated with a horned phenotype in a bovine subject comprising
a. determining a haplotype in at least one allele of at least one horned bovine subject, wherein the haplotype allele comprises the nucleotide occurrence of a nucleotide polymorphism corresponding to nucleotide position 300 of each of SEQ ID NOs: - 53, 59, 52, 63, 62, and 61,
b. thereby, identifying a haplotype allele associated the horned phenotype in a bovine subject. - View Dependent Claims (17, 18)
- 53, 59, 52, 63, 62, and 61,
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19. A method for identifying a haplotype allele associated with a polled phenotype in a bovine subject comprising:
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a. detecting the nucleotide occurrence of single nucleotide polymorphisms corresponding to nucleotide position 300 of each of SEQ ID NOs;
53, 59, 52, 63, 62, and 61, in both first and second alleles of a first bovine subject, wherein the first subject displays the polled phenotype;b. identifying the horned or polled phenotype of a second bovine subject, wherein the second subject is homozygous for the first haplotype allele identified in step a., wherein a polled phenotype of the second subject identifies the first haplotype allele as a haplotype allele associated with the polled phenotype, and wherein a horned phenotype of the second subject identifies the second haplotype allele as a haplotype allele associated with the polled phenotype, thereby, identifying a haplotype allele associated the polled phenotype in a bovine subject. - View Dependent Claims (20)
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Specification