Method of combing a nucleic acid

• US 20110195870A1
• Filed: 12/20/2010
• Published: 08/11/2011
• Est. Priority Date: 06/30/2006
• Status: Active Grant

• Current Assignee: Enterprise Science Fund, LLC (Intellectual Ventures LLC)
• Original Assignee: The Invention Science Fund I, L.L.C. (Intellectual Ventures LLC)
• Inventors: Wood, Lowell L. JR., Hyde, Roderick A.
• Granted Patent: US 8,628,913 B2
• Time in Patent Office: Days
• US Class Current: 506/30
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/0038 Drawing
• CPC Class Codes: B01J 2219/00382 Stamping
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00727 Glycopeptides
• CPC Class Codes: B01J 2219/00734 Lipids
• CPC Class Codes: B01J 2219/00756 Compositions, e.g. coatings, crystals, formulations
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 1/6841 : In situ hybridisation
• CPC Class Codes: C12Q 2565/629 : being a microfluidic device
• CPC Class Codes: H10K 71/191 : characterised by provisions for the orientation or alignment of the layer to be deposited
• CPC Class Codes: H10K 85/761 : Biomolecules or bio-macromolecules, e.g. proteins, chlorophyl, lipids or enzymes

Abstract

Elongated molecules are stretched across a substrate by controlled fluid flow.

Claims

1. A method of forming a nanowire, comprising:

applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature thereon;

controllably flowing the nucleic acid-containing solution over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate; and

applying a conductive material to the stretched nucleic acid molecule to form a nanowire.

2. The method of claim 1, wherein the nucleic acid includes DNA.

4. The method of claim 1, wherein the nucleic acid includes RNA.

6. The method of claim 1, wherein the nucleic acid includes a synthetic nucleic acid.

8. The method of claim 1, wherein the nucleic acid molecule includes a molecule selected from the group consisting of chromosomes, viruses, plasmids, oligonucleotides, double-stranded nucleic acids, and single-stranded nucleic acids.

9. The method of claim 1, wherein the nucleic acid is bound to a nanotube.

10. The method of claim 1, wherein the defined local physical feature is a depression.

11. The method of claim 1, wherein the defined local physical feature is a protrusion.

12. The method of claim 1, wherein the first substrate has a plurality of defined local physical features, and wherein the controlled fluid flow promotes stretching of multiple nucleic acid molecules from at least a subset of the plurality of defined local physical features along the first substrate.

13. The method of claim 12, wherein the plurality of defined local physical features are arranged in a repeating array.

36. The method of claim 1, wherein the conductive material includes a metal.

37. The method of claim 1, wherein the conductive material includes a semiconductor.

38. The method of claim 1, wherein the conductive material includes a compound.

39. The method of claim 1, wherein the conductive material includes a polymer.

40. The method of claim 1, wherein the conductive material is substantially contiguous.

61. The method of claim 1, wherein the first substrate comprises surface features that spatially direct the fluid flow.

62. The method of claim 1, wherein the first substrate comprises a plurality of micro-orifices, and wherein controllably flowing further comprises flowing a fluid through at least a subset of the micro-orifices.

63. The method of claim 62, wherein the fluid that flows through the at least a subset of the micro-orifices is the nucleic acid-containing solution.

64. The method of claim 62, wherein the fluid that flows through the at least a subset of the micro-orifices is a gas.

65. The method of claim 1, wherein the first substrate comprises a plurality of switchable channels, and wherein controllably flowing further comprises switching at least a subset of the switchable channels.

3. (canceled)

5. (canceled)

7. (canceled)

14. A method of forming a nanowire, comprising:

applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature thereon;

controllably flowing the nucleic acid-containing solution over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate;

applying a conductive material to the stretched nucleic acid molecule to form a nanowire; and

transferring the stretched nucleic acid molecule to a second substrate.

15. The method of claim 14, wherein the stretched nucleic acid molecule is transferred before applying the conductive material.

16. The method of claim 14, wherein the stretched nucleic acid molecule is transferred after applying the conductive material.

17. The method of claim 14, wherein transferring the stretched nucleic acid molecule comprises bringing the first substrate into contact with the second substrate.

19. The method of claim 14, further comprising controlling a surface charge of the second substrate, wherein the surface charge acts to attract the nucleic acid molecule to the second substrate.

20. The method of claim 19, further comprising controlling a surface charge of the first substrate, wherein the surface charge acts to attract the nucleic acid molecule to the first substrate.

21. The method of claim 19, wherein controlling a surface charge of the second substrate includes inducing a spatiotemporally patterned surface charge on the second substrate.

22. The method of claim 14, further comprising controlling a surface charge of the first substrate, wherein the surface charge acts to repel the nucleic acid molecule from the first substrate.

23. The method of claim 22, further comprising controlling a surface charge of the second substrate, wherein the surface charge acts to attract the nucleic acid molecule to the second substrate.

24. The method of claim 22, further comprising controlling a surface charge of the second substrate, wherein the surface charge acts to repel the nucleic acid molecule from the second substrate.

25. The method of claim 22, wherein controlling a surface charge of the first substrate includes inducing a spatiotemporally patterned surface charge on the first substrate.

29. The method of claim 14, wherein transferring the stretched nucleic acid molecule comprises controlling surface energy of at least one of the first and second substrates.

30. The method of claim 14, wherein transferring the stretched nucleic acid molecule comprises controlling temperature of at least one of the first and second substrates.

31. The method of claim 14, wherein transferring the stretched nucleic acid molecule comprises controlling hydrophilicity of at least one of the first and second substrates.

32. The method of claim 14, wherein the second substrate comprises an electronic device, and wherein the transferred nucleic acid molecule is in electrical communication with the electronic device.

33. The method of claim 14, further comprising attaching an electronic device to the transferred nucleic acid molecule.

18. (canceled)

26-28. -28. (canceled)

34. (canceled)

35. (canceled)

41-60. -60. (canceled)

66-83. -83. (canceled)

84. A method of applying a nucleic acid to a surface, comprising:

applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature thereon, the defined local physical feature comprising a nucleic acid anchor; and

controllably flowing a deposition fluid over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate.

85. The method of claim 84, wherein the deposition fluid is the nucleic-acid containing solution.

91. The method of claim 84, wherein the nucleic acid anchor comprises an anchor strand of nucleic acid attached to the first substrate.

93. The method of claim 84, wherein the nucleic acid-containing solution comprises a nucleic acid molecule bound to a mechanical anchor, and wherein the nucleic acid anchor comprises a trap for the mechanical anchor.

94. The method of claim 93, wherein the mechanical anchor is a bead.

97. The method of claim 93, wherein the trap physically traps the mechanical anchor.

99. The method of claim 93, wherein the trap adheres to the mechanical anchor.

100. The method of claim 93, wherein the trap electrically traps the mechanical anchor.

101. The method of claim 93, wherein the trap magnetically traps the mechanical anchor.

110. The method of claim 84, wherein the defined local physical feature is a depression.

111. The method of claim 84, wherein the defined local physical feature is a protrusion.

112. The method of claim 84, wherein the first substrate has a plurality of defined local physical features, and wherein the controlled fluid flow promotes stretching of multiple nucleic acid molecules from at least a subset of the plurality of defined local physical features along the first substrate.

113. The method of claim 112, wherein the plurality of defined local physical features are arranged in a repeating array.

86-90. -90. (canceled)

92. (canceled)

95-96. -96. (canceled)

98. (canceled)

102-109. -109. (canceled)

114. A method of applying a nucleic acid to a surface, comprising:

applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature thereon, the defined local physical feature comprising a nucleic acid anchor;

controllably flowing a deposition fluid over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate; and

transferring the stretched nucleic acid molecule to a second substrate.

115-163. -163. (canceled)