METHOD AND SYSTEM FOR DETECTING FLUOROCHROMES IN A FLOW CYTOMETER
First Claim
1. A method for detecting fluorochromes in a flow cytometer, comprising:
- receiving a sample including particles each tagged with at least one of a first fluorochrome and a second fluorochrome, wherein the first and second fluorochromes have distinct spillover coefficients;
detecting particles of the sample, including detecting the first and second fluorochromes of the particles with a first detector and a second detector;
forming a data set for detected particles based on the detection of the first and second fluorochromes of the particles;
characterizing a detected spillover coefficient for each detected fluorochrome from the data set; and
sorting the detected particles into predicted fluorochrome populations based on the detected spillover coefficients.
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Abstract
A method for detecting fluorochromes in a flow cytometer, including: receiving a sample including particles each tagged with at least one of a first fluorochrome and a second fluorochrome, in which the first and second fluorochromes having distinct spillover coefficients; detecting the particles, including detecting the first and second fluorochromes with a first detector and a second detector; forming a data set for detected particles based on the detection of the first and second fluorochromes; characterizing a detected spillover coefficient for each detected fluorochrome from the data set; and sorting the detected particles into predicted fluorochrome populations based on the detected spillover coefficients. A system for detecting fluorochromes in a flow cytometer, including a flow cell, a fixed gain detection system, and a processor that generates a detected spillover coefficient for each detected particle and sorts the detected particle into predicted fluorochrome populations based on the detected spillover coefficient.
110 Citations
30 Claims
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1. A method for detecting fluorochromes in a flow cytometer, comprising:
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receiving a sample including particles each tagged with at least one of a first fluorochrome and a second fluorochrome, wherein the first and second fluorochromes have distinct spillover coefficients; detecting particles of the sample, including detecting the first and second fluorochromes of the particles with a first detector and a second detector; forming a data set for detected particles based on the detection of the first and second fluorochromes of the particles; characterizing a detected spillover coefficient for each detected fluorochrome from the data set; and sorting the detected particles into predicted fluorochrome populations based on the detected spillover coefficients. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. A system for detecting fluorochromes in a flow cytometer, comprising:
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an interrogation zone that receives a sample including particles tagged with at least one of a first fluorochrome and a second fluorochrome, wherein the first and second fluorochromes have distinct spillover coefficients; a detection system that detects particles passing through the flow cell, by detecting the first and second fluorochromes of the particles, and forms a data set based on detection of the first and second fluorochromes, respectively; and a processor that generates a detected spillover coefficient for each detected particle from the data set and sorts the detected particles into predicted fluorochrome populations based on the detected spillover coefficient. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30)
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Specification