Devices and Methods for Tissue Engineering

• US 20110206828A1
• Filed: 03/11/2011
• Published: 08/25/2011
• Est. Priority Date: 07/10/2009
• Status: Abandoned Application

• Current Assignee: Bio2 Technologies, Inc.
• Original Assignee: Bio2 Technologies, Inc.
• Inventors: Liu, James Jeng, Krevolin, Janet L.
• Application Number:
  US13/046,192
• Publication Number:
  US 20110206828A1
• Time in Patent Office: Days
• US Class Current: 427/2.24
• CPC Class Codes: A61F 2/28 Bones joints A61F2/30
• CPC Class Codes: A61F 2/3094 Designing or manufacturing processes
• CPC Class Codes: A61F 2/4465 having a circular or kidney shaped cross-section substantially perpendicular to the axis of the spine
• CPC Class Codes: A61F 2002/30062 (bio)absorbable, biodegradable, bioerodable, (bio)resorbable, resorptive
• CPC Class Codes: A61F 2002/3092 having an open-celled or open-pored structure
• CPC Class Codes: A61F 2002/30968 Sintering
• CPC Class Codes: A61F 2002/4495 having a fabric structure, e.g. made from wires or fibres
• CPC Class Codes: A61F 2210/0004 bioabsorbable
• CPC Class Codes: A61F 2310/00329 Glasses, e.g. bioglass
• CPC Class Codes: A61L 24/0036 Porous materials, e.g. foams or sponges
• CPC Class Codes: A61L 24/02 containing inorganic materials
• CPC Class Codes: A61L 2400/18 Modification of implant surfaces in order to improve biocompatibility, cell growth, fixation of biomolecules, e.g. plasma treatment
• CPC Class Codes: A61L 2430/02 for reconstruction of bones; weight-bearing implants
• CPC Class Codes: A61L 27/10 Ceramics or glasses
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: C03C 11/00 Multi-cellular glass ; Porous or hollow glass or glass particles
• CPC Class Codes: C03C 4/0014 Biodegradable glass

Abstract

A resorbable tissue scaffold fabricated from bioactive glass fiber forms a rigid three-dimensional porous matrix having a bioactive composition. Porosity in the form of interconnected pore space is provided by the space between the bioactive glass fiber in the porous matrix. Strength of the bioresorbable matrix is provided by bioactive glass that fuses and bonds the bioactive glass fiber into the rigid three-dimensional matrix. The resorbable tissue scaffold supports tissue in-growth to provide osteoconductivity as a resorbable tissue scaffold, used for the repair of damaged and/or diseased bone tissue.

Claims

1. A method of fabricating a porous tissue scaffold comprising:

providing glass fibers having a bioactive composition, the glass fibers having a diameter in the range of about 1 to 200 μ

m;

providing a bonding agent comprising particles having a bioactive composition;

providing a binder;

providing a pore former having a particle size in the range of about 0.1 to about 100 times the diameter of the glass fibers;

mixing the glass fibers, the bonding agent, the binder, and the pore former to provide a homogeneous mixture;

directing the mixture into a cavity;

forming the mixture into a solidified shaped object using the cavity;

removing the binder and the pore former from the shaped object; and

bonding the glass fibers into a porous structure using the bonding agent.

2. The method according to claim 1 wherein the steps of directing the mixture into a cavity and forming the mixture into a solidified shape comprise an injection molding process.

3. The method according to claim 2 wherein the step of forming the mixture into a solidified shaped object comprises cooling the mixture.

4. The method according to claim 3 wherein the step of directing the homogeneous mixture into a cavity comprises heating the mixture to change the phase of the binder from a solid to at least partially a liquid.

5. The method according to claim 1 wherein the steps of directing the homogeneous mixture into a cavity and forming the mixture into a solidified shaped object comprises a compression molding process.

6. The method according to claim 1 wherein the steps of directing the homogeneous mixture into a cavity and forming the mixture into a solidified shaped object comprises a slip casting process.

7. The method according to claim 6 further comprising a step of providing a liquid, and wherein the step of forming the mixture into a solidified shaped object using the cavity comprises at least partially removing the liquid.

8. The method according to claim 1 further comprising a finishing step to remove at least a portion of the porous structure to define a dimension of the porous tissue engineering scaffold.

9. A method of fabricating a porous tissue engineering scaffold comprising:

providing fiber having a bioactive composition;

providing a bonding agent;

providing a binder;

providing a pore former;

mixing the fiber, the bonding agent, the binder, and the pore former into a homogeneous mixture;

forming the mixture into a solidified shaped object;

removing the binder and the pore former; and

heat treating the shaped object to bond the bioactive fiber into a porous structure using the bonding agent, the porous structure having interconnected porosity.

10. The method according to claim 9 wherein the step of forming the mixture into a solidified shaped object comprises an extrusion process.

11. The method according to claim 9 wherein the step of forming the mixture into a solidified shaped object comprises a wet layup accumulation of the mixture.

12. The method according to claim 9 wherein the step of forming the mixture into a solidified shaped object comprises dispensing a plurality of portions of the mixture.

13. The method according to claim 9 further comprising a finishing step to remove at least a portion of the porous structure to define a dimension of the porous tissue engineering scaffold.

14. A method of fabricating a porous coating on an orthopedic implant, the method comprising:

providing an orthopedic implant;

providing fiber having a bioactive composition;

providing a bonding agent;

providing a binder;

providing a pore former;

mixing the fiber, the bonding agent, the binder, and the pore former into a homogeneous mixture;

forming the mixture into a solidified shaped object on at least a portion of the orthopedic implant;

removing the binder and the pore former; and

heat treating the shaped object to bond the bioactive fiber and the bonding agent into a porous structure and simultaneously bonding at least a portion of the bioactive fiber and the bonding agent to the orthopedic implant, the porous structure having interconnected porosity.

15. The method according to claim 14 wherein the step of forming the mixture into a solidified shaped object on at least a portion of the orthopedic implant comprises extruding the mixture over the orthopedic implant.

16. The method according to claim 14 wherein the step of forming the mixture into a solidified shaped object on at least a portion of the orthopedic implant comprises injection molding.

17. The method according to claim 14 wherein the step of forming the mixture into a solidified shaped object on at least a portion of the orthopedic implant comprises compression molding.

18. The method according to claim 14 wherein the step of forming the mixture into a solidified shaped object on at least a portion of the orthopedic implant comprises a wet layup accumulation of the mixture.

19. The method according to claim 14 wherein the step of forming the mixture into a solidified shaped object on at least a portion of the orthopedic implant comprises dispensing a plurality of portions of the mixture.

20. The method according to claim 14 further comprising a finishing step to remove at least a portion of the porous structure to define a dimension of the porous tissue engineering scaffold.