MICROFLUIDIC SYSTEM FOR AMPLIFYING AND DETECTING POLYNUCLEOTIDES IN PARALLEL
First Claim
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1. An apparatus, comprising:
- a plurality of multi-lane microfluidic cartridges, each lane comprising a PCR reaction zone;
a plurality of receiving bays, each receiving bay configured to receive one of the plurality of microfluidic cartridges;
each PCR reaction zone comprising a separately controllable heat source thermally coupled thereto, wherein the heat source maintains a substantially uniform temperature throughout the PCR reaction zone and thermal cycles the PCR reaction zone to carry out PCR on a polynucleotide-containing sample in the PCR reaction zone;
a detector configured to detect the presence of an amplification product in one or more PCR reaction zones; and
a processor coupled to the detector and the heat sources, configured to control heating of one or more PCR reaction zones by the heat sources.
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Abstract
The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.
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Citations
20 Claims
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1. An apparatus, comprising:
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a plurality of multi-lane microfluidic cartridges, each lane comprising a PCR reaction zone; a plurality of receiving bays, each receiving bay configured to receive one of the plurality of microfluidic cartridges; each PCR reaction zone comprising a separately controllable heat source thermally coupled thereto, wherein the heat source maintains a substantially uniform temperature throughout the PCR reaction zone and thermal cycles the PCR reaction zone to carry out PCR on a polynucleotide-containing sample in the PCR reaction zone; a detector configured to detect the presence of an amplification product in one or more PCR reaction zones; and a processor coupled to the detector and the heat sources, configured to control heating of one or more PCR reaction zones by the heat sources. - View Dependent Claims (2, 3, 4, 5)
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6. A device for carrying out PCR on a plurality of samples, the device comprising:
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a plurality of multi-lane microfluidic cartridges, each lane comprising a PCR reaction zone; a plurality of receiving bays, each receiving bay configured to receive one of the plurality of microfluidic cartridges; a separately controllable heat source thermally coupled to each PCR reaction zone, wherein the heat source is configured to maintain a substantially uniform temperature throughout the PCR reaction zone and thermal cycle the PCR reaction zone to carry out PCR on a polynucleotide-containing sample in the PCR reaction zone; a detector configured to detect the presence of an amplification product in one or more PCR reaction zones; and a processor coupled to the detector and a plurality of the separately controllable heat sources, configured to control heating of one or more PCR reaction zones by one or more of the plurality of separately controllable heat sources; an input device coupled to the processor and configured to permit concurrent or consecutive control of the plurality of multi-lane microfluidic cartridges. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A method of carrying out PCR on a plurality of samples, the method comprising:
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introducing the plurality of samples into a plurality of multi-lane microfluidic cartridges, wherein each lane comprises a PCR reaction zone configured to permit thermal cycling of a sample independently of the other samples; moving the plurality of samples into the respective plurality of PCR reaction zones; and amplifying polynucleotides contained with the plurality of samples in the plurality of PCR reaction zones while thermal cycling the PCR reaction zones, at least one PCR reaction zone separately thermally controllable from another PCR reaction zone. - View Dependent Claims (19, 20)
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Specification