IN VITRO SCREENING ASSAYS
First Claim
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1. A method for identifying an inhibitor of LOXL2 activity, the method comprising:
- (a) providing a co-culture of endothelial cells growing in the presence of stromal cells;
(b) adding a test molecule to the co-culture; and
(c) assaying the co-culture for angiogenesis or vasculogenesis;
wherein a test molecule that reduces the degree of angiogenesis or vasculogenesis in the co-culture, compared to a co-culture in the absence of the test molecule, is identified as an inhibitor of LOXL2 activity.
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Abstract
Disclosed herein are methods for identifying inhibitors of the catalytic activity of LOXL2 using in vitro assays. In certain embodiments, inhibitors identified by these in vitro assays are effective in inhibiting tumor desmoplasia and fibrosis.
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15 Claims
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1. A method for identifying an inhibitor of LOXL2 activity, the method comprising:
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(a) providing a co-culture of endothelial cells growing in the presence of stromal cells; (b) adding a test molecule to the co-culture; and (c) assaying the co-culture for angiogenesis or vasculogenesis; wherein a test molecule that reduces the degree of angiogenesis or vasculogenesis in the co-culture, compared to a co-culture in the absence of the test molecule, is identified as an inhibitor of LOXL2 activity. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification