ARRANGEMENT FOR PROCESSING A PLURALITY OF SAMPLES FOR ANALYSIS

• US 20110207619A1
• Filed: 11/29/2007
• Published: 08/25/2011
• Est. Priority Date: 12/05/2006
• Status: Abandoned Application

• Current Assignee: Siemens AG
• Original Assignee: Siemens AG
• Inventors: Schonecke, Mitja, Zilch, Christian, Ehben, Thomas
• Application Number:
  US12/448,015
• Publication Number:
  US 20110207619A1
• Time in Patent Office: Days
• US Class Current: 506/9
• CPC Class Codes: B01L 2300/021 Identification, e.g. bar codes
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: G01N 35/04 Details of the conveyor system G01N35/021 - G01N35/028 take precedence

Abstract

An arrangement is provided in at least one embodiment, having a magazine for supplying a plurality of microfluidic devices. The microfluidic devices each contain at least one element/device for binding at least one biological molecule, wherein the at least one element/device for binding the at least one biological molecule can be moved relative to the microfluidic device. A sample presumably containing biological molecules to be examined is introduced into the microfluidic device. The biological molecule to be examined is bound by the at least one element/device for binding the biological molecule. In at least one embodiment, the at least one element/device for binding the at least one biological molecule, or the substrate-molecule complex, can then be moved in the microfluidic device, e.g. in accordance with a predetermined reaction sequence, for example by means of a magnetic field. The microfluidic device is transported through the arrangement.

Claims

1. An arrangement for processing a plurality of samples for analysis, the arrangement comprising:

a receptacle for a microfluidic device, including means for binding at least one biological molecule provided in the microfluidic device;

means for moving the microfluidic device in the arrangement along at least one direction of movement; and

at least one magazine for supplying a plurality of microfluidic devices.

2. The arrangement as claimed in claim 1, further comprising:

a unit for moving means, provided in the microfluidic device, for binding at least one biological molecule relative to the microfluidic device.

3. The arrangement as claimed in claim 2, wherein the unit for moving means provided in the microfluidic device for binding at least one biological molecule relative to the microfluidic device comprises a magnetic field generator.

4. The arrangement as claimed in claim 1, further comprising:

means for amplifying the biological molecule in the microfluidic device.

5. The arrangement as claimed in claim 1, further comprising:

means for detecting the biological molecule.

6. The arrangement as claimed in claim 1, further comprising:

a unit for introducing a sample into a microfluidic device.

7. The arrangement as claimed in claim 1, further comprising:

a container for collecting used microfluidic devices.

8. The arrangement as claimed in claim 1, wherein the at least one magazine is a stack magazine, in which the microfluidic devices are stackable.

9. The arrangement as claimed in claim 1, wherein the at least one magazine is a drum magazine, in which the microfluidic devices are rollable on a roll.

10. The arrangement as claimed in claim 1, further comprising:

means for detecting a coding of a microfluidic device.

11. A method for processing a plurality of samples for analysis, the method comprising:

a) supplying an arrangement with a plurality of microfluidic devices, wherein the arrangement has at least one magazine for supply with microfluidic devices and wherein the microfluidic devices each contain at least one element for binding at least one biological molecule;

b) introducing a first sample, containing at least one biological molecule to be examined, into one of the microfluidic devices;

c) binding the biological molecule to be examined by the at least one element for binding at least one biological molecule; and

d) repeating steps b)-c) with the further samples until all the samples to be processed have been processed,

wherein the microfluidic device with the introduced sample is moved in the arrangement along at least one direction of movement.

12. The method as claimed in claim 11, wherein the at least one element means for binding the at least one biological molecule is movable relative to the microfluidic device.

13. The method as claimed in claim 11, wherein the introduction of the samples, containing biological molecules to be examined, into the respective microfluidic devices is effected before the supply of the arrangement with microfluidic devices.

14. The method as claimed in claim 11, wherein the at least one element for binding the at least one biological molecule have a substrate that can be linked to the molecule to form a substrate-molecule complex.

15. The method as claimed in claim 14, wherein, after the binding of the molecule to the substrate, the substrate-molecule complex is separated from the rest of the sample.

16. The method as claimed in claim 15, wherein the separation of the substrate-molecule complex from the rest of the sample is effected by moving the substrate-molecule complex relative to the rest of the sample.

17. The method as claimed in claim 11, wherein the at least one element for binding the at least one biological molecule comprise at least one magnetic element.

18. The method as claimed in claim 17, wherein a magnetic field is used for moving the at least one element for binding the at least one biological molecule relative to the microfluidic device.

19. The method as claimed in claim 11, further comprising:

amplifying the biological molecule by way of an amplification reaction.

20. The method as claimed in claim 11, further comprising:

detecting the biological molecule.

21. The method as claimed in claim 11, wherein the at least one element for binding the at least one molecule is moved along a reaction section in the microfluidic device, which leads into at least one process chamber.

22. The method as claimed in claim 21, wherein the at least one element for binding the at least one molecule is moved along a reaction section in the microfluidic device through a plurality of process chambers.

23. The method as claimed in claim 21, wherein the reaction section is oriented essentially along the at least one direction of movement in the arrangement.

24. The method as claimed in claim 21, wherein, in the microfluidic device, a plurality of samples are processed simultaneously in a corresponding number of reaction sections which are arranged essentially parallel in the microfluidic device.

29. The method as claimed in claim 12, wherein the introduction of the samples, containing biological molecules to be examined, into the respective microfluidic devices is effected before the supply of the arrangement with microfluidic devices.

30. The method as claimed in claim 12, wherein the at least one element for binding the at least one biological molecule have a substrate that can be linked to the molecule to form a substrate-molecule complex.

31. The method as claimed in claim 22, wherein the reaction section is oriented essentially along the at least one direction of movement in the arrangement.

25-28. -28. (canceled)

32. An arrangement for processing a plurality of samples for analysis, the arrangement comprising:

a receptacle for a microfluidic device, including at least one element to bind at least one biological molecule provided in the microfluidic device;

at least one device to move the microfluidic device in the arrangement along at least one direction of movement; and

at least one magazine to supply a plurality of microfluidic devices.

33. The arrangement as claimed in claim 32, further comprising:

a unit for moving device, provided in the microfluidic device, to bind at least one biological molecule relative to the microfluidic device.

34. The arrangement as claimed in claim 32, further comprising:

at least one device to amplify the biological molecule in the microfluidic device.

35. The arrangement as claimed in claim 32, further comprising:

at least one device to detect the biological molecule.

36. The arrangement as claimed in claim 32, wherein the at least one magazine is a stack magazine, in which the microfluidic devices are stackable.

37. The arrangement as claimed in claim 32, wherein the at least one magazine is a drum magazine, in which the microfluidic devices are rollable on a roll.