Glycosylation of Molecules
First Claim
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1. A method of producing an altered N-glycosylation form of a target protein, the method comprising:
- providing a Yarrowia lipolytica or an Arxula adeninivorans cell genetically engineered to comprise a deficiency in Outer CHain elongation (OCH1) activity; and
introducing into the cell a nucleic acid encoding a target protein, wherein the cell produces the target protein in an altered N-glycosylation form relative to the N-glycosylation form of the target protein produced in a corresponding non-genetically engineered cell.
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Abstract
Described herein are methods and genetically engineered cells useful for producing an altered N-glycosylation form of a target molecule. Also described are methods and molecules with altered N-glycosylation useful for treating a variety of disorders such as metabolic disorders.
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Citations
40 Claims
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1. A method of producing an altered N-glycosylation form of a target protein, the method comprising:
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providing a Yarrowia lipolytica or an Arxula adeninivorans cell genetically engineered to comprise a deficiency in Outer CHain elongation (OCH1) activity; and introducing into the cell a nucleic acid encoding a target protein, wherein the cell produces the target protein in an altered N-glycosylation form relative to the N-glycosylation form of the target protein produced in a corresponding non-genetically engineered cell. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 31)
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- 24. An isolated Yarrowia lipolytica or Arxula adeninivorans cell genetically engineered to comprise a deficiency in OCH1 activity.
- 32. A method of producing an altered N-glycosylation form of a target protein, the method comprising contacting a target protein with a cell lysate prepared from a Yarrowia lipolytica or an Arxula adeninivorans cell genetically engineered to comprise a deficiency in OCH1 activity, wherein the contacting of the target protein with the cell lysate results in an altered N-glycosylation form of the target protein relative to the N-glycosylation form of the target protein produced from the cell lysate of a corresponding non-genetically engineered cell.
- 36. A substantially pure culture of Yarrowia lipolytica or Arxula adeninivorans cells, a substantial number of which are genetically engineered to comprise a deficiency in OCH1 activity.
Specification