SYSTEMS AND METHODS FOR PRESUMPTIVE IDENTIFICATION OF MICROORGANISM TYPE IN A CULTURE
First Claim
1. A method of identifying a microorganism type in a culture in a vessel, the method comprising:
- (A) calculating a normalization relative value for each respective measurement in a plurality of measurements of a biological state of the culture in the vessel, taken at different time points between a first time point and a second time point, between (i) the respective measurement and (ii) an initial biological state of the culture taken at an initial time point, thereby forming a plurality of normalization relative values;
(B) determining, for each respective predetermined fixed interval of time points between the first time point and the second time point, a first derivative of the normalization relative values for measurements of the biological state in the respective predetermined fixed interval of time points, thereby forming a plurality of rate transformation values, wherein the plurality of rate transformation values comprises a plurality of sets of rate transformation values, wherein each respective set of rate transformation values in the plurality of sets of rate transformation values is for a different set of contiguous time points between the first time point and the second time point;
(C) computing, for each respective set of rate transformation values in the plurality of sets of rate transformation values, an average relative transformation value as a measure of central tendency of each of the rate transformation values in the respective set of rate transformation values, thereby computing a plurality of average relative transformation values;
(D) determining a maximum metabolic rate and an extent of growth from the plurality of normalization relative values and the plurality of average relative transformation values; and
(E) determining the microorganism type in the culture in the vessel from the maximum metabolic rate and the extent of growth.
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Abstract
Systems, methods and apparatus for identifying a microorganism type in a culture are provided. A normalization relative value is calculated for each respective measurement of a biological state of the culture between (i) the respective measurement and (ii) an initial biological state. For each fixed interval of time points, a first derivative of the normalization relative values in the interval of time points is calculated, thereby forming a plurality of rate transformation values. For each respective set of rate transformation values in the plurality of rate transformation values, a measure of central tendency of the rate transformation values in the set is computed, thereby forming a plurality of average relative transformation values. A maximum metabolic rate and an extent of growth, determined from the normalization relative values and the average relative transformation values, are compared against a lookup table that matches these values to a microorganism type.
6 Citations
56 Claims
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1. A method of identifying a microorganism type in a culture in a vessel, the method comprising:
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(A) calculating a normalization relative value for each respective measurement in a plurality of measurements of a biological state of the culture in the vessel, taken at different time points between a first time point and a second time point, between (i) the respective measurement and (ii) an initial biological state of the culture taken at an initial time point, thereby forming a plurality of normalization relative values; (B) determining, for each respective predetermined fixed interval of time points between the first time point and the second time point, a first derivative of the normalization relative values for measurements of the biological state in the respective predetermined fixed interval of time points, thereby forming a plurality of rate transformation values, wherein the plurality of rate transformation values comprises a plurality of sets of rate transformation values, wherein each respective set of rate transformation values in the plurality of sets of rate transformation values is for a different set of contiguous time points between the first time point and the second time point; (C) computing, for each respective set of rate transformation values in the plurality of sets of rate transformation values, an average relative transformation value as a measure of central tendency of each of the rate transformation values in the respective set of rate transformation values, thereby computing a plurality of average relative transformation values; (D) determining a maximum metabolic rate and an extent of growth from the plurality of normalization relative values and the plurality of average relative transformation values; and (E) determining the microorganism type in the culture in the vessel from the maximum metabolic rate and the extent of growth. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48)
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49. An apparatus for identifying a microorganism type in a culture in a vessel, the apparatus comprising a processor and a memory, coupled to the processor, the memory comprising:
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a microorganism type determination module comprising; (A) electronically encoded instructions for calculating a normalization relative value for each respective measurement in a plurality of measurements, taken at a different time points between a first time point and a second time point, between (i) the respective measurement and (ii) an initial biological state of the culture taken at an initial time point, thereby forming a plurality of normalization relative values; (B) electronically encoded instructions for determining, for each respective predetermined fixed interval of time points between the first time point and the second time point, a first derivative of the normalization relative values for measurements of the biological state in the respective predetermined fixed interval of time points, thereby forming a plurality of rate transformation values, wherein the plurality of rate transformation values comprises a plurality of sets of rate transformation values, wherein each respective set of rate transformation values in the plurality of sets of rate transformation values is for a different set of contiguous time points between the first time point and the second time point; (C) electronically encoded instructions for computing, for each respective set of rate transformation values in the plurality of sets of rate transformation values, an average relative transformation value as a measure of central tendency of each of the rate transformation values in the respective set of rate transformation values, thereby computing a plurality of average relative transformation values; (D) electronically encoded instructions for determining a maximum metabolic rate and an extent of growth from the plurality of normalization relative values and the plurality of average relative transformation values; and (E) electronically encoded instructions for determining the microorganism type in the culture in the vessel from the maximum metabolic rate and the extent of growth. - View Dependent Claims (50)
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51. A computer-readable medium storing a computer program product for identifying a microorganism in a culture in a vessel, executable by a computer, wherein the computer program product comprises:
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a microorganism type determination module comprising; (A) electronically encoded instructions for calculating a normalization relative value for each respective measurement in a plurality of measurements, taken at a different time points between a first time point and a second time point, between (i) the respective measurement and (ii) an initial biological state of the culture taken at an initial time point, thereby forming a plurality of normalization relative values; (B) electronically encoded instructions for determining, for each respective predetermined fixed interval of time points between the first time point and the second time point, a first derivative of the normalization relative values for measurements of the biological state in the respective predetermined fixed interval of time points, thereby forming a plurality of rate transformation values, wherein the plurality of rate transformation values comprises a plurality of sets of rate transformation values, wherein each respective set of rate transformation values in the plurality of sets of rate transformation values is for a different set of contiguous time points between the first time point and the second time point; (C) electronically encoded instructions for computing, for each respective set of rate transformation values in the plurality of sets of rate transformation values, an average relative transformation value as a measure of central tendency of each of the rate transformation values in the respective set of rate transformation values, thereby computing a plurality of average relative transformation values; (D) electronically encoded instructions for determining a maximum metabolic rate and an extent of growth from the plurality of normalization relative values and the plurality of average relative transformation values; and (E) electronically encoded instructions for determining the microorganism type in the culture in the vessel from the maximum metabolic rate and the extent of growth. - View Dependent Claims (52)
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53. An apparatus for identifying a microorganism type in a culture in a vessel, the apparatus comprising a processor and a memory, coupled to the processor, the memory comprising:
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(A) electronically encoded instructions for obtaining a plurality of sets of values, each set of values in the plurality of sets of values comprising a maximum metabolic rate value and an extent of growth for a different microorganism type in a set of microorganism types; and (B) electronically encoded instructions for storing a lookup table that comprises matches between (i) the plurality of sets of values and (ii) a set of microorganism types, wherein, for each set of values in the plurality of sets of values there is a corresponding microorganism type in the set of microorganism types. - View Dependent Claims (54)
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55. A method of identifying a microorganism type in a culture in a vessel, the method comprising:
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(A) obtaining a plurality of measurements of the biological state of the culture in the vessel, each measurement in the plurality of measurements taken at a different time point between a first time point and a second time point; (B) determining, for each respective predetermined fixed interval of time points between the first time point and the second time point, a first derivative of the measurements of the biological state in the respective predetermined fixed interval of time points, thereby forming a plurality of rate transformation values, wherein the plurality of rate transformation values comprises a plurality of sets of rate transformation values, wherein each respective set of rate transformation values in the plurality of sets of rate transformation values is for a different set of contiguous time points between the first time point and the second time point; (C) computing, for each respective set of rate transformation values in the plurality of sets of rate transformation values, an average relative transformation value as a measure of central tendency of each of the rate transformation values in the respective set of rate transformation values, thereby computing a plurality of average relative transformation values; (D) determining a maximum metabolic rate and an extent of growth from the plurality of normalization relative values and the plurality of average relative transformation values; and (E) determining the microorganism type in the culture in the vessel from the maximum metabolic rate and the extent of growth. - View Dependent Claims (56)
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Specification