ANTI-PAF ANTIBODIES
First Claim
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1. An isolated monoclonal antibody, or an antigen binding fragment thereof, that binds platelet activating factor (PAF), wherein the isolated antibody or antigen binding fragment thereof optionally comprises at least one heavy chain variable region and at least one light chain variable region, wherein each light chain variable region comprises at least one of the following light chain CDRs:
- (i) a CDRL1 comprising the amino acid sequence ITTTDIKRNMN (SEQ ID NO;
3) or a variant thereof in which from 1 to 10 amino acids residues are substituted with a different amino acid residue;
(ii) a CDRL2 comprising the amino acid sequence QGNILRP (SEQ ID NO;
4) or a variant thereof in which from 1 to 6 amino acids residues are substituted with a different amino acid residue; and
(iii) a CDRL3 comprising the amino acid sequence LQSRGLPFT (SEQ ID NO;
5) or a variant thereof in which from 1 to 8 amino acids residues are substituted with a different amino acid residue.
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Abstract
Monoclonal antibodies to platelet activating factor (PAF) are described, along with methods for their production and use. Such antibodies can be formulated and used for therapeutic purposes, as well as for diagnosis and detection.
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Citations
11 Claims
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1. An isolated monoclonal antibody, or an antigen binding fragment thereof, that binds platelet activating factor (PAF), wherein the isolated antibody or antigen binding fragment thereof optionally comprises at least one heavy chain variable region and at least one light chain variable region, wherein each light chain variable region comprises at least one of the following light chain CDRs:
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(i) a CDRL1 comprising the amino acid sequence ITTTDIKRNMN (SEQ ID NO;
3) or a variant thereof in which from 1 to 10 amino acids residues are substituted with a different amino acid residue;(ii) a CDRL2 comprising the amino acid sequence QGNILRP (SEQ ID NO;
4) or a variant thereof in which from 1 to 6 amino acids residues are substituted with a different amino acid residue; and(iii) a CDRL3 comprising the amino acid sequence LQSRGLPFT (SEQ ID NO;
5) or a variant thereof in which from 1 to 8 amino acids residues are substituted with a different amino acid residue. - View Dependent Claims (2, 3, 4, 5, 6, 10, 11)
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- 7. A diagnostic reagent comprising a derivatized platelet activating factor (PAF), wherein said derivatized PAF comprises a polar head group and at least one hydrocarbon chain, wherein a carbon atom within a hydrocarbon chain is derivatized with a reactive group, wherein the reactive group optionally is a sulfhydryl (thiol) group, and wherein the derivatized PAF optionally is (i) associated with a solid support, wherein the association optionally is a covalent association, or (ii) conjugated to a carrier moiety, wherein the carrier moiety optionally is selected from the group consisting of polyethylene glycol, colloidal gold, adjuvant, a silicone bead, and a protein, wherein the protein is optionally selected from the group consisting of keyhole limpet hemocyanin, albumin, ovalbumin, bovine thyroglobulin, and soybean trypsin inhibitor.
Specification