MOLECULAR REDUNDANT SEQUENCING

• US 20110212436A1
• Filed: 02/03/2011
• Published: 09/01/2011
• Est. Priority Date: 07/26/2007
• Status: Active Grant

• Current Assignee: Pacific Bioscience of California Incorporated (L'Oreal SA)
• Original Assignee: Pacific Bioscience of California Incorporated (L'Oreal SA)
• Inventors: Turner, Stephen, Christians, Fred
• Granted Patent: US 8,535,882 B2
• Time in Patent Office: Days
• US Class Current: 435/6.1
• CPC Class Codes: C12Q 1/6869 Methods for sequencing

Abstract

Methods, systems and compositions where a target nucleic acid includes a registration sequence disposed therein for identification of the number or relative position of determined sequence from the template sequence. Particularly preferred aspects include a registration sequence in a circular template nucleic acid sequence which is, in turn, used in sequence by incorporation processes that rely upon template dependent, polymerase mediated primer extension in the identification of the sequence of the template.

Claims

1-30. -30. (canceled)

31. A method of obtaining a consensus sequence for an individual nucleic acid molecule, comprising:

sequencing a first sequence of nucleotides present in the individual nucleic acid molecule a plurality of times to obtain a plurality of base calls at each position of the first sequence; and

calling bases within the sequence of the first nucleic acid based upon at least a 75% consensus among the plurality of base calls for each position of the sequence of the first nucleic acid.

32. The method of claim 31, comprising calling bases within the sequence of the first nucleic acid based upon at least an 80% consensus among the plurality of base calls for each position of the sequence of the first nucleic acid.

33. The method of claim 31, comprising calling bases within the sequence of the first nucleic acid based upon at least a 90% consensus among the plurality of base calls for each position of the sequence of the first nucleic acid.

34. The method of claim 31, wherein the first sequence of nucleotides is sequenced at least 2 times.

35. The method of claim 31, wherein the first sequence of nucleotides is sequenced at least 5 times.

36. The method of claim 31, wherein the first sequence of nucleotides is sequenced at least 10 times.

37. The method of claim 31, wherein the first sequence of nucleotides is sequenced at least 15 times.

38. The method of claim 31, wherein the first sequence of nucleotides is sequenced at least 20 times.

39. The method of claim 31, wherein the individual nucleic acid molecule comprises a circular nucleic acid molecule.

40. The method of claim 31, wherein the first sequence of nucleotides is sequenced by detecting polymerase mediated nucleotide addition in a template directed primer extension reaction.