LABELS FOR ELECTRONIC DETECTION OF INDIVIDUAL MOLECULES AND METHODS FOR THEIR DETECTION
First Claim
1. A label for the electronic detection of individual target analyte molecules comprising a target specific probe capable of binding the target analyte molecule attached to a polymeric molecule or filament, wherein the polymeric molecule or filament comprises one or more elements with different electrical properties as detected by a nanoelectrode and the elements with different electrical properties are not detected by changes in fluorescence.
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Abstract
The invention provides labels for electronic detection of individual molecules. The labels are comprised of elements with different electrical properties that affect the electric current flowing through a nanoelectrode. The labels are of polymeric or filamentous structure where the elements are arranged linearly along their length. The arrangement of the elements is predetermined and combinatorial, so that a high diversity of labels can be generated in a manner that resembles barcoding on a nanoscale level. Methods for the synthesis of said barcode labels and for the binding of the barcode labels to individual molecules, their movement past a nanoelectrode, and their detection are also provided.
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Citations
48 Claims
- 1. A label for the electronic detection of individual target analyte molecules comprising a target specific probe capable of binding the target analyte molecule attached to a polymeric molecule or filament, wherein the polymeric molecule or filament comprises one or more elements with different electrical properties as detected by a nanoelectrode and the elements with different electrical properties are not detected by changes in fluorescence.
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16. A composition comprising two or more labels, each of which comprises a target specific probe capable of binding a target analyte molecule attached to a polymeric molecule or filament wherein the polymeric molecule or filament comprises one or more elements with different electrical properties as detected by a nanoelectrode, and wherein the elements with different electrical properties are not detected by changes in fluorescence and the target specific probe of each label is different from each of the other labels.
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17. A kit for the electronic detection of individual target analyte molecules comprising:
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(a) one or more labels each of which comprises a target specific probe capable of binding the target analyte molecule attached to a polymeric molecule or filament which comprises at least two elements with different electrical properties; and (b) a nanoelectrode capable of detecting the elements with different electrical properties. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 48)
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30-47. -47. (canceled)
Specification