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SYSTEM AND METHOD TO CORRECT OUT OF PHASE ERRORS IN DNA SEQUENCING DATA BY USE OF A RECURSIVE ALGORITHM

  • US 20110213563A1
  • Filed: 03/08/2011
  • Published: 09/01/2011
  • Est. Priority Date: 02/15/2007
  • Status: Active Grant
First Claim
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1. A method for recursively correcting an error associated with phasic synchrony of sequence data generated from a population of substantially identical copies of a template molecule, comprising:

  • (a) detecting a plurality of signals generated in response to a plurality of nucleotide species introduced during a sequencing reaction;

    (b) generating an observed value for the signal detected from each of the nucleotide species;

    (c) defining a plurality of positive incorporation values and a plurality of negative incorporation values from the observed values using a carry forward value and an incomplete extension value;

    (d) revising the carry forward value and the incomplete extension value using a noise value, wherein the noise value is derived from a plurality of observed values associated with the negative incorporation values;

    (e) re-defining the plurality of positive incorporation values and the plurality of negative incorporation values using the revised carry forward value and the revised incomplete extension value; and

    (f) repeating steps (d)-(e) until convergence of the plurality of positive incorporation values and the plurality of negative incorporation values.

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