SINGLE-MOLECULE DETECTION SYSTEM AND METHODS
First Claim
Patent Images
1. A single-molecule detection system, comprising:
- a) a movable light coupler;
b) a waveguide comprising;
a core layer, anda first cladding layer,wherein at least one adapter site for the movable light coupler is formed in at least the first cladding layer; and
c) a light detector.
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Abstract
Embodiments encompass a single-molecule detection system and methods of using the detection system to detect an object. Further, embodiments encompass a detection system comprising a movable light coupler, a waveguide, and a light detector. Embodiments further encompass methods of single-molecule detection, including methods of single-molecule nucleic acid sequencing.
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Citations
36 Claims
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1. A single-molecule detection system, comprising:
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a) a movable light coupler; b) a waveguide comprising; a core layer, and a first cladding layer, wherein at least one adapter site for the movable light coupler is formed in at least the first cladding layer; and c) a light detector. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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34. A method of detecting a single-molecule object, comprising:
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a) introducing an incident light from a light source into a waveguide, thereby forming an excitation light in the waveguide; b) localizing a single-molecule object on a movable light coupler, c) localizing the movable light coupler of (b) at an adapter site for a movable light coupler formed in at least a first cladding layer of the waveguide; and d) exciting, by the excitation light, the single-molecule object localized on the movable light coupler, causing the single-molecule object to emit a light to be detected by a light detector.
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35. A method of detecting a single-molecule object, comprising:
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a) providing a detection apparatus comprising i) a movable light coupler; ii) a waveguide comprising; a core layer, and a first cladding layer, wherein at least one adapter site for the movable light coupler is formed in at least the first cladding layer; and iii) a light detector; b) providing at least one binding moiety capable of binding a single-molecule object; c) localizing the at least one binding moiety individually on the movable light coupler; d) providing a single-molecule object molecule to the at least one binding moiety localized on the movable light coupler; e) localizing at the adapter site the movable light coupler on which the at least one binding moiety and single-molecule object are localized; f) introducing an incident light from a light source into the waveguide, thereby forming an excitation light in the waveguide; h) exciting, by the excitation light, the single-molecule object molecule bound to the at least one binding moiety localized on the movable light coupler, causing the single-molecule object to emit a light to be detected by a light detector.
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36. A method of sequencing a nucleic acid, comprising:
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a) providing a detection apparatus comprising i) a movable light coupler; ii) a waveguide comprising; a core layer, and a first cladding layer, wherein at least one adapter site for the movable light coupler is formed in at least the first cladding layer; and iii) a light detector; b) providing at least one nucleic acid molecule; c) localizing the at least one nucleic acid molecule individually on the movable light coupler; d) localizing at the adapter site the movable light coupler on which the at least one nucleic acid is localized; e) performing single molecule sequencing-by-synthesis of the at least one nucleic acid molecule, wherein the single molecule nucleic acid sequencing-by-synthesis leads produces an emitted light correlated to the identity of at least one base in the nucleic acid; f) detecting the emitted light with the detector, resulting in an output signal; and g) processing the output signal to determine an identity of at least one base comprised by the nucleic acid.
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Specification