Multiple-sample microfluidic chip for DNA analysis
First Claim
1. A microfluidic chip, comprising:
- a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, the first domain including at least a first reaction reservoir designated for PCR amplification based on a first sample, and a second reaction reservoir designated for PCR amplification based on a second sample; and
a second domain including at least a first separation unit coupled to the first reaction reservoir to received first amplified DNA fragments based on the first sample, and a second separation unit coupled to the second reaction reservoir to received second amplified DNA fragments based on the second sample, the first separation unit being configured to perform electrophoretic separation for the first amplified DNA fragments, and the second separation unit being configured to perform electrophoretic separation for the second amplified DNA fragments.
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Abstract
Aspects of the disclosure provide a microfluidic chip. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, and a second domain for electrophoretic separation. The first domain includes at least a first reaction reservoir designated for PCR amplification based on a first sample, and a second reaction reservoir designated for PCR amplification based on a second sample. The second domain includes at least a first separation unit coupled to the first reaction reservoir to received first amplified DNA fragments based on the first sample, and a second separation unit coupled to the second reaction reservoir to received second amplified DNA fragments based on the second sample. The first separation unit is configured to perform electrophoretic separation for the first amplified DNA fragments, and the second separation unit is configured to perform electrophoretic separation for the second amplified DNA fragments.
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Citations
28 Claims
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1. A microfluidic chip, comprising:
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a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, the first domain including at least a first reaction reservoir designated for PCR amplification based on a first sample, and a second reaction reservoir designated for PCR amplification based on a second sample; and a second domain including at least a first separation unit coupled to the first reaction reservoir to received first amplified DNA fragments based on the first sample, and a second separation unit coupled to the second reaction reservoir to received second amplified DNA fragments based on the second sample, the first separation unit being configured to perform electrophoretic separation for the first amplified DNA fragments, and the second separation unit being configured to perform electrophoretic separation for the second amplified DNA fragments. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A cartridge, comprising:
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a sample acceptor configured to respectively extract a first template DNA from a first sample and a second template DNA from a second sample; and a microfluidic chip having a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, the first domain including at least a first reaction reservoir designated for PCR amplification based on the first template DNA, and a second reaction reservoir designated for PCR amplification based on the second template DNA; and a second domain including at least a first separation unit coupled to the first reaction reservoir to received first amplified DNA fragments based on the first template DNA, and a second separation unit coupled to the second reaction reservoir to received second amplified DNA fragments based on the second template DNA, the first separation unit being configured to perform electrophoretic separation for the first amplified DNA fragments, and the second separation unit being configured to perform electrophoretic separation for the second amplified DNA fragments. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A method for multiple-sample DNA analysis, comprising:
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inducing thermal cycles in a first domain of a microfluidic chip for PCR amplification of DNA fragments, the first domain including at least a first reaction reservoir designated for PCR amplification based on a first sample, and a second reaction reservoir designated for PCR amplification based on a second sample; inducing liquid flow to respectively move first amplified DNA fragments from the first reaction reservoir to a first separation unit in a second domain of the microfluidic chip, and second amplified DNA fragments from the second reaction reservoir to a second separation unit in the second domain of the microfluidic chip; inducing electric fields in the first separation unit to separate the first amplified DNA fragments by size; inducing electric fields in the second separation unit to separate the second amplified DNA fragments by size; and detecting the separated DNA fragments. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28)
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Specification