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INTRACELLULAR pH IMAGING METHOD AND APPARATUS USING FLURESCENCE LIFETIME

  • US 20110224512A1
  • Filed: 03/09/2011
  • Published: 09/15/2011
  • Est. Priority Date: 03/10/2010
  • Status: Abandoned Application
First Claim
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1. A pH measurement method comprising the steps of:

  • generating pulsed excitation light including a wavelength that can excite a predetermined fluorescent material contained in living matter, the fluorescent material acting as coenzyme in oxidation/reduction reaction in vivo, and the intensity of the pulsed excitation light not damaging a tissue nor a cell in the living matter and substantially not changing the pH of the living matter;

    illuminating a predetermined position in the living matter with the pulsed excitation light;

    receiving light including fluorescence emitted from the fluorescent material excited by illumination with the pulsed excitation light;

    calculating the lifetime of the fluorescence by time-resolving the intensity of the received fluorescence; and

    measuring the pH of the living matter based on the lifetime.

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