NANOFLUIDIC CHANNELS WITH INTEGRATED CHARGE SENSORS AND METHODS BASED THEREON
First Claim
1. An electrical detector comprising a nanofluidic channel and a charge sensor, wherein:
- the charge sensor is selected from the group consisting of nanowire, nanotube, transistor and capacitor, anda portion of the charge sensor is integrated in the nanofluidic channel, whereby the charge sensor is contacted by fluid in the nanofluidic channel.
3 Assignments
0 Petitions
Accused Products
Abstract
An electrical detector is provided that comprises a nanofluidic channel with an integrated nanoscale charge sensor. The charge sensor can be an unfunctionalized nanowire, nanotube, transistor or capacitor and can be of carbon, silicon, carbon/silicon or other semiconducting material. The nanofluidic channel depth is on the order of the Debye screening length. Methods are also provided for detecting charged molecules or biological or chemical species with the electrical detector. Charged molecules or species in solution are driven through the nanofluidic channel of the electrical detector and contact the charge sensor, thereby producing a detectable signal. Methods are also provided for detecting a local solution potential of interest. A solution flowing through the nanofluidic channel of the electrical detector contacts the charge sensor, thereby producing a detectable local solution potential signal.
180 Citations
69 Claims
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1. An electrical detector comprising a nanofluidic channel and a charge sensor, wherein:
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the charge sensor is selected from the group consisting of nanowire, nanotube, transistor and capacitor, and a portion of the charge sensor is integrated in the nanofluidic channel, whereby the charge sensor is contacted by fluid in the nanofluidic channel. - View Dependent Claims (4, 5, 9, 12, 15, 16, 19, 21, 28, 30, 31, 33, 43, 45, 49, 58, 64, 68)
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2-3. -3. (canceled)
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6-8. -8. (canceled)
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10-11. -11. (canceled)
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13-14. -14. (canceled)
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17-18. -18. (canceled)
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20. (canceled)
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22-27. -27. (canceled)
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29. (canceled)
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32. (canceled)
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34-42. -42. (canceled)
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44. (canceled)
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46-48. -48. (canceled)
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50-57. -57. (canceled)
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59-63. -63. (canceled)
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65-66. -66. (canceled)
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67. A method for measuring conformation, length, speed or label intensity of a charged molecule or particle of interest comprising:
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providing; a charged molecule or particle of interest labeled with an optically detectable label (or feature, tag or modification), a nanoslit, a nanofluidic channel, and two spatially separated focal volumes defined by lasers focused sequentially on the nanochannel; driving electrophoretically the labeled charged molecule or particle from the nanoslit into the nanofluidic channel; transporting the labeled charged molecule or particle through the two spatially separated focal volumes, thereby generating a first optically detectable signal and a second optically detectable signal shifted in time relative to each other; detecting photon bursts (or photon count signals) from the first and second optically detectable signals; measuring the photon bursts (or photon count signals) from the first and second optically detectable signals; performing a speed or a cross correlation measurement using the measurement from the photon bursts (or photon count signals) from the first and second optically detectable signals; and calculating the conformation, length, speed or label intensity of the molecule or particle of interest from the speed or cross correlation measurement.
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69-73. -73. (canceled)
Specification