MICROORGANISMS WITH INACTIVATED LACTATE DEHYDROGENASE GENE (LDH) FOR CHEMICAL PRODUCTION
First Claim
Patent Images
1. An isolated microorganism genetically modified to lack expression of a wild-type lactate dehydrogenase gene, wherein the microorganism can hydrolyze both C5 and C6 polysaccharides.
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Abstract
This invention provides systems and methods for the increased production of ethanol and other chemical compounds by recombinant Clostridium species whereby the recombinant species are genetically-engineered to disrupt lactate dehydrogenase activity and to hydrolyze and ferment carbonaceous biomass and synthesize compounds of commercial value without production of lactic acid.
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Citations
51 Claims
- 1. An isolated microorganism genetically modified to lack expression of a wild-type lactate dehydrogenase gene, wherein the microorganism can hydrolyze both C5 and C6 polysaccharides.
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10. An isolated microorganism selected from the group consisting of:
- NRRL Accession No. NRRL B 50351, NRRL B-50352, NRRL B-50353, NRRL B 50354, NRRL B-50355, NRRL B-50356, NRRL B-50357, NRRL B-50358, and NRRL B-50359.
- 11. An isolated mesophilic microorganism that lacks lactate dehydrogenase activity, wherein said mesophilic microorganism ferments polysaccharides.
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12. An isolated mesophilic microorganism, genetically modified to produce higher amounts of ethanol, wherein said modification comprises inactivation of lactic acid synthesis as it occurs in the wild-type mesophilic microorganism.
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16. A product for production of a chemical product comprising:
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a. a fermentation vessel comprising a carbonaceous biomass; and b. a microorganism genetically modified to inactivate a lactate dehydrogenase gene of the wild-type organism, wherein the microorganism hydrolyzes both C5 and C6 polysaccharides and produces a chemical product. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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28. A process for producing a chemical product comprising:
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a. contacting a carbonaceous biomass with a microorganism genetically modified to inactivate a lactate dehydrogenase gene of the wild-type organism, wherein the microorganism hydrolyzes both C5 and C6 polysaccharides; b. allowing sufficient time for said hydrolysis and fermentation to produce the chemical product. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42)
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- 43. An isolated microorganism that ferments cellulosic or lignocellulosic materials to produce ethanol in a concentration that is at least 90% of a theoretical yield, wherein the microorganism is genetically modified to inactivate a lactate dehydrogenase gene of the wild-type organism.
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48. A plasmid comprising SEQ ID NO:
- 1.
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49. A primer selected from the group comprising SEQ ID NO:
- 2, SEQ ID NO;
3, SEQ ID NO;
4, and SEQ ID NO;
5.
- 2, SEQ ID NO;
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50. A plasmid comprising a nucleic acid sequence with about 70% similarity to SEQ ID NO:
- 1.
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51. A primer comprising a nucleic acid sequence with about 70% similarity to SEQ ID NO:
- 2, SEQ ID NO;
3, SEQ ID NO;
4, or SEQ ID NO;
5.
- 2, SEQ ID NO;
Specification