APPARATUS AND METHODS FOR INTEGRATED SAMPLE PREPARATION, REACTION AND DETECTION
First Claim
Patent Images
1. An apparatus, comprising:
- an isolation module including a first housing and a second housing, the first housing defining a first chamber and a second chamber, at least the first chamber configured to contain a sample, the second housing including a side wall and a puncturable member, the side wall and the puncturable member collectively defining a first volume configured to contain a first substance, a portion of the second housing configured to be disposed within the first housing such that the first volume is in fluid communication with the first chamber when a portion of the puncturable member is punctured; and
a reaction module defining a reaction chamber and a second volume configured to contain a second substance, the reaction module configured to be coupled to the isolation module such that the reaction chamber and the second volume are each in fluid communication with the second chamber of the first housing.
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Abstract
Cartridges for the isolation of a biological sample and downstream biological assays on the sample are provided. In one embodiment, a nucleic acid sample is isolated from a biological sample and the nucleic acid sample is amplified, for example by the polymerase chain reaction. The cartridges provided herein can also be used for the isolation of non-nucleic acid samples, for example proteins, and to perform downstream reactions on the proteins, for example, binding assays. Instruments for carrying out the downstream biological assays and for detecting the results of the assays are also provided.
153 Citations
28 Claims
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1. An apparatus, comprising:
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an isolation module including a first housing and a second housing, the first housing defining a first chamber and a second chamber, at least the first chamber configured to contain a sample, the second housing including a side wall and a puncturable member, the side wall and the puncturable member collectively defining a first volume configured to contain a first substance, a portion of the second housing configured to be disposed within the first housing such that the first volume is in fluid communication with the first chamber when a portion of the puncturable member is punctured; and a reaction module defining a reaction chamber and a second volume configured to contain a second substance, the reaction module configured to be coupled to the isolation module such that the reaction chamber and the second volume are each in fluid communication with the second chamber of the first housing. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. An apparatus, comprising:
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a first module defining a first chamber and a second chamber, the first module including a first transfer mechanism configured to transfer a sample between the first chamber and the second chamber while maintaining fluid isolation between the first chamber and the second chamber; a second module defining a volume configured to contain a substance, a portion of the second module configured to be disposed within the first chamber of the first module when the second module is coupled to the first module such that the volume is configured to be selectively placed in fluid communication with the first chamber; and a third module defining a reaction chamber, the third module configured to be coupled to the first module such that the reaction chamber is in fluid communication with the second chamber, the third module including a second transfer mechanism configured to transfer a portion of the sample between the second chamber and the reaction chamber. - View Dependent Claims (16, 17, 18, 19, 20, 21)
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22. An apparatus, comprising:
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a first module including a reaction vial, a substrate and a first transfer mechanism, the reaction vial defining a reaction chamber, the substrate defining at least a portion of a first flow path and a second flow path, the first flow path configured to be in fluid communication with the reaction chamber and an isolation chamber of an isolation module, the first transfer mechanism configured to transfer a sample from the isolation chamber to the reaction chamber when the first transfer mechanism is actuated; and a second module including a second transfer mechanism and defining a volume configured to contain a substance, the second module configured to be coupled to the first module such that the volume can be selectively placed in fluid communication with the reaction chamber via the second flow path, the second transfer mechanism configured to transfer the substance from the volume to the reaction chamber when the second transfer mechanism is actuated. - View Dependent Claims (23, 24, 25, 26, 27, 28)
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Specification