RNA INTERFERENCE IN SKIN INDICATIONS
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Accused Products
Abstract
The present invention relates to RNAi constructs with improved tissue and cellular uptake characteristics and methods of use of these compounds in dermal applications.
157 Citations
41 Claims
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1-2. -2. (canceled)
- 3. A method for treating compromised skin, the method comprising, administering to a subject in need thereof a therapeutically effective amount for treating compromised skin of a double stranded nucleic acid molecule comprising a guide strand, with a minimal length of 16 nucleotides, and a passenger strand forming a double stranded nucleic acid, having a double stranded region and a single stranded region, the double stranded region having 8-15 nucleotides in length, the single stranded region having 4-12 nucleotides in length, wherein the single stranded region comprises 2-12 phosphorothioate modifications, and wherein at least 40% of the nucleotides of the double stranded nucleic acid are modified.
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4. A method comprising,
administering a double stranded nucleic acid molecule selected from the nucleic acid molecules contained in Tables 1-3, or a combination thereof, such that an antisense and a sense strand make up the double stranded nucleic acid molecule, to a subject in need thereof.
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5-6. -6. (canceled)
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7. A method for treating or preventing a fibrotic disorder in a subject in need thereof, the method comprising:
administering to the subject a therapeutically effective amount for treating or preventing a fibrotic disorder of a double stranded nucleic acid molecule comprising a guide strand, with a minimal length of 16 nucleotides, and a passenger strand forming a double stranded nucleic acid, having a double stranded region and a single stranded region, the double stranded region having 8-15 nucleotides in length, the single stranded region having 4-12 nucleotides in length, wherein the single stranded region comprises 2-12 phosphorothioate modifications, and wherein at least 40% of the nucleotides of the double stranded nucleic acid are modified. - View Dependent Claims (25, 36, 37, 38)
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9-12. -12. (canceled)
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14-17. -17. (canceled)
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19-24. -24. (canceled)
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26-29. -29. (canceled)
- 30. A composition, comprising a double stranded nucleic acid molecule comprising a guide strand, with a minimal length of 16 nucleotides, and a passenger strand forming a double stranded nucleic acid, having a double stranded region and a single stranded region, the double stranded region having 8-15 nucleotides in length, the single stranded region having 4-12 nucleotides in length, wherein the single stranded region comprises 2-12 phosphorothioate modifications, wherein at least 40% of the nucleotides of the double stranded nucleic acid are modified, and wherein the composition is formulated for delivery to the skin.
Specification