IMPLANTABLE MEDICAL ARTICLES HAVING LAMININ COATINGS AND METHODS OF USE

• US 20110244014A1
• Filed: 01/31/2011
• Published: 10/06/2011
• Est. Priority Date: 02/23/2005
• Status: Abandoned Application

• Current Assignee: David E. Babcock, David L. Clapper, Joseph A. Chinn, Stuart K. Williams
• Original Assignee: David E. Babcock, David L. Clapper, Joseph A. Chinn, Stuart K. Williams
• Inventors: Clapper, David L., Chinn, Joseph A., Williams, Stuart K., Babcock, David E.
• Application Number:
  US13/018,038
• Publication Number:
  US 20110244014A1
• Time in Patent Office: Days
• US Class Current: 424/422
• CPC Class Codes: A61K 38/10 Peptides having 12 to 20 amino acids A61K38/043 - A61K38/046 take precedence
• CPC Class Codes: A61K 38/39 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
• CPC Class Codes: A61L 2300/25 Peptides having up to 20 amino acids in a defined sequence
• CPC Class Codes: A61L 2300/254 Enzymes, proenzymes
• CPC Class Codes: A61L 2300/606 Coatings
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 29/085 Macromolecular materials
• CPC Class Codes: A61L 31/10 Macromolecular materials
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00
• CPC Class Codes: C08L 89/06 derived from leather or skin , e.g. gelatin

Abstract

Laminin-containing coatings for the surfaces of implantable medical devices are disclosed. The coatings promote the formation of vessels in association with the coated surfaces with minimal fibrotic response.

Claims

1. An implantable medical article having a coating comprising

a first component comprising laminin, an active portion thereof, or a binding member thereof, and

a second component comprising collagen I or collagen IV, an active portion thereof, or a binding member thereof,

wherein the coating further comprises a polymeric component, a first group reacted to form a layer comprising the polymeric component, and second groups reacted to individually bond first and second components to the polymeric component,

wherein upon implantation in a subject, the coating enhances adhesion of endothelial cells to the implantable medical article.

2. The implantable medical article of claim 1 wherein the first component comprises laminin having a molecular weight of less than 500 kDa.

3. The implantable medical article of claim 1 wherein the first component comprises laminin-5.

4. The implantable medical article of claim 1 wherein the first component comprises the α

3 chain of laminin-5.

5. The implantable medical article of claim 1 wherein the first component comprises the LG3 sequence of the α

3 chain of laminin-5.

6. The implantable medical article of claim 1 wherein the first component comprises a laminin polypeptide sequence selected from PPFLMLLKGSTR (Pro Pro Phe Leu Met Leu Leu Lys Gly Ser Thr Arg;

SEQ ID NO.;

1), LAIKNDNLVYVY (Leu Ala Ile Lys Asn Asp Asn Leu Val Tyr Val Tyr;

SEQ ID NO.;

4), DVISLYNFKHIY (Asp Val Ile Ser Leu Tyr Asn Phe Lys His Ile Tyr;

SEQ ID NO.;

5), TLFLAHGRLVFM (Thr Leu Phe Leu Ala His Gly Arg Leu Val Phe Met;

SEQ ID NO.;

6), LVFMFNVGHKKL (Leu Val Phe Met Phe Asn Val Gly His Lys Lys Leu;

SEQ ID NO.;

7), and NSFMALYLSKGR (Asn Ser Phe Met Ala Leu Tyr Leu Ser Lys Gly Arg;

SEQ ID NO.;

2).

7. The implantable medical article of claim 1 wherein the first component comprises proteinase-modified laminin-5.

8. The implantable medical article of claim 7 wherein the first component comprises metalloproteinase-modified laminin-5.

9. The implantable medical article of claim 1 wherein the first component comprises laminin-1.

10. The implantable medical article of claim 1 comprising a porous portion.

11. The implantable medical article of claim 10 wherein the porous portion is associated with a graft, sheath, or jacket.

12. The implantable medical article of claim 10 wherein the porous portion comprises a synthetic hydrophobic polymeric material.

13. The implantable medical article of claim 12 wherein the porous portion comprises ePTFE.

14. The implantable medical article of claim 1 wherein the polymeric component comprises a synthetic polymer.

15. The implantable medical article of claim 14 wherein the synthetic polymer is an acrylamide polymer.

16. The implantable medical article of claim 1 wherein the first group comprises a photoreactive group.

17. The implantable medical article of claim 1 wherein the second group comprises an amine-reactive group.

18. An implantable medical article having a coating comprising

a first component comprising laminin, an active portion thereof, or a binding member thereof, and

a second component comprising an adhesion factor, an active portion thereof, or a binding member thereof,

wherein the coating further comprises a polymeric component, a first group reacted to form a layer comprising the polymeric component, and second groups reacted to individually bond first and second components to the polymeric component.

19. An implantable medical article comprising a stably denucleated porous portion.

20. The implantable medical article of claim 19 wherein the porous portion comprises a coated layer comprising a synthetic polymer.