METHODS FOR CONDUCTING CELLULAR ASSAYS

• US 20110250632A1
• Filed: 12/16/2009
• Published: 10/13/2011
• Est. Priority Date: 12/18/2008
• Status: Abandoned Application

• Current Assignee: GE Healthcare UK Limited (Danaher Corporation)
• Original Assignee: GE Healthcare UK Limited (Danaher Corporation)
• Inventors: Tatnell, Peter James, Game, Stephen, Doyle, Anne Michelle
• Application Number:
  US13/140,056
• Publication Number:
  US 20110250632A1
• Time in Patent Office: Days
• US Class Current: 435/29
• CPC Class Codes: A01N 1/0221 Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents

Abstract

The present invention relates to cryopreserved cell cultures, methods for cryopreserving cells and methods for conducting cellular assays on such cells. A cryopreserved cell culture of the invention comprises a container having at least a surface which is coated with poly-lysine and frozen cells supported on the surface.

Claims

1. A cryopreserved cell culture, comprising:

a container having at least a surface;

frozen adherent cells supported on said surface; and

cryopreservation medium;

wherein the surface is coated with poly-lysine.

3. The cell culture of claim 1, wherein said container is selected from the group consisting of vessel, vial, microtitre plate and cell culture plate.

6. The cell culture of claim 1, wherein the cells are mammalian cells.

2. (canceled)

4-5. -5. (canceled)

7. (canceled)

8. A method of cryopreserving cells in a cell culture, said method comprising the steps of:

a) adding a medium containing adherent cells to a surface of a container to allow said cells to attach to said surface and form a cell culture;

b) adding a cryopreservation medium; and

c) reducing the temperature of said cell culture to −

20°

C. or below;

characterized by coating the surface with poly-lysine prior to step a).

10. The method of claim 8, wherein the surface is coated by washing the surface with a solution of poly-lysine.

11. The method of claim 8, wherein the container is selected from the group consisting of vessel, vial, microtitre plate and cell culture plate.

14. The method of claim 8, wherein the cells are mammalian cells.

16. The method of claim 8, further comprising the step of storing the cell culture at a temperature below −

80°

C.

9. (canceled)

12-13. -13. (canceled)

15. (canceled)

17. A method for conducting a cellular assay, said method comprising the steps of:

a) adding a medium containing adherent cells to a surface of a container to allow said cells to attach to said surface and form a cell culture;

b) adding a cryopreservation medium;

c) reducing the temperature of said medium to freeze said cell culture;

d) storing the cell culture at a temperature of less than −

20°

C.;

e) thawing said cells by raising the temperature of the cell culture; and

f) conducting a cellular assay on the cells,

characterized by coating the surface of said container with poly-lysine prior to step a).

18. The method of claim 17, wherein the cell culture is stored at a temperature of less than −

80°

C.

20. The method of claim 17, wherein the container is selected from the group consisting of vessel, vial, microtitre plate and cell culture plate.

22. The method of claim 17, wherein the cells of step a) are attached to one or more micro-carriers.

23. The method of claim 22, wherein said one or more micro-carriers are coated with poly-lysine.

25. The method of claim 17, wherein the cells are mammalian cells.

19. (canceled)

21. (canceled)

24. (canceled)

26. (canceled)