ASSAY FOR TROPONIN I USING MAGNETIC LABELS

• US 20110256640A1
• Filed: 12/15/2009
• Published: 10/20/2011
• Est. Priority Date: 12/22/2008
• Status: Active Grant

• Current Assignee: Siemens Healthcare Nederland B.V. (Siemens AG)
• Original Assignee: Koninklijke Philips Electronics N.V. (Koninklijke Philips N.V.)
• Inventors: Kamps, Ricky, De Kievit, Peggy, Vissers, Joost Lambert Max, Dekkers, David Walterus Cornelis, Martens, Michael Franciscus Wilhelmus Cornelis, Dittmer, Wendy Uyen, Evers, Toon Hendrik
• Granted Patent: US 9,720,003 B2
• Time in Patent Office: Days
• US Class Current: 436/501
• CPC Class Codes: G01N 2800/324 Coronary artery diseases, e.g. angina pectoris, myocardial infarction
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/6887 from muscle, cartilage or connective tissue

Abstract

The present invention relates to a method for measuring Troponin I in a sample comprising the steps of providing a sample, contacting the sample with a monoclonal anti-Troponin I antibody coupled to a magnetic label, contacting the sample with a polyclonal anti-Troponin I antibody coupled to a sensor surface and detecting the magnetic label on the sensor surface. The invention further relates to a device and a cartridge for measuring Troponin I in a sample.

Claims

1. Method for measuring Troponin I in a sample comprising at least the following steps:

a. Providing a sample

b. Contacting the sample with a monoclonal anti-Troponin I antibody coupled to a magnetic label

c. Contacting the sample with a polyclonal anti-Troponin I antibody coupled to a sensor surface, or at least two different anti-Troponin I antibodies coupled to a sensor surface

d. Detecting the magnetic label on the sensor surface, wherein steps b and c can be performed in any order.

2. Method according to claim 1, wherein step b precedes step c.

3. Method according to claim 1, wherein the magnetic label is a particle comprising inorganic material or inorganic and organic material.

4. Method according to claim 1, wherein the magnetic label has a size of 200 to 1000 nm.

5. Method according to claim 1, wherein the anti-Troponin I antibody bound to a magnetic label not complexed to the sensor surface is removed from the sensor surface by using a magnetic field and magnetic field gradient.

6. Method according to claim 1, wherein the polyclonal anti-Troponin I antibody used is a goat polyclonal antibody.

7. Method according to claim 1 wherein the magnetic label is optically detected.

8. Method according to claim 1 wherein the magnetic label is magnetically detected.

9. Biosensor device capable of measuring Troponin I according to the method according to claim 1.

14. Use of the method according to claim 1 in the process of diagnosing myocardial infarction.

10. A cartridge for use in an assay device, comprising:

a. a monoclonal anti-Troponin I antibody bound to a magnetic label

b. a polyclonal anti-Troponin I antibody coupled to a sensor surface, or at least two different anti-Troponin I antibodies coupled to a sensor surface, within the cartridge, and

c. a sample inlet.

11. The cartridge according to claim 10, wherein the magnetic label is a particle comprising inorganic material or inorganic and organic material.

12. The cartridge according to claim 10 wherein the magnetic label has a size of 200 to 1000 nm.

13. The cartridge according to claim 10 wherein the polyclonal anti-Troponin I antibody is a goat polyclonal antibody.